Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0TZT0 (TRMB_PHANO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
ORF Names:SNOG_15087
OrganismPhaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum) [Reference proteome]
Taxonomic identifier321614 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePhaeosphaeriaceaeParastagonospora

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370601

Regions

Region153 – 1542S-adenosyl-L-methionine binding By similarity
Region255 – 2573S-adenosyl-L-methionine binding By similarity

Sites

Active site1761 By similarity
Binding site1041S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1731S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TZT0 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: FC6F4361519E15A2

FASTA28232,547
        10         20         30         40         50         60 
MPHAPAKRQK REEYKNALHE DESNAALPKK KFYRQRAHAN PFSDHSLTYP KSPADMDWAS 

        70         80         90        100        110        120 
LYPAYAVVKR EQKSAGEEES TPLDEEEQRR LKAITKNVEI ADIGCGFGGL LFALAPKFPD 

       130        140        150        160        170        180 
TLMLEYVQEK VRALRLQNAS IQLYQNASCL RANTMKFLPN FFSKAQLSKI FLCFPDPHFK 

       190        200        210        220        230        240 
QRKHKARIVS YTLNSEYAYV LRPGGVVYTI TDVKDLHEWM VGHFEKHPSF ERCEKEFEEE 

       250        260        270        280 
GEAMDEGVGI MRTETEEGKK VSRNGGMKYV ACFRRVEDPE WP 

« Hide

References

[1]"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum."
Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P.
Plant Cell 19:3347-3368(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH445359 Genomic DNA. Translation: EAT77630.2.
RefSeqXP_001805250.1. XM_001805198.1.

3D structure databases

ProteinModelPortalQ0TZT0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSNOT_15087; SNOT_15087; SNOG_15087.
GeneID5982178.
KEGGpno:SNOG_15087.

Phylogenomic databases

KOK03439.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PHANO
AccessionPrimary (citable) accession number: Q0TZT0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways