Q0TZT0 (TRMB_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	SNOG_15087

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum) [Reference proteome]

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › dothideomyceta › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria › Phaeosphaeria nodorum

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370601
Regions
	Region	153 – 154	2	S-adenosyl-L-methionine binding By similarity
	Region	255 – 257	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	176	1	By similarity
	Binding site	104	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	173	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TZT0 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: FC6F4361519E15A2	FASTA	282	32,547
	10 20 30 40 50 60 MPHAPAKRQK REEYKNALHE DESNAALPKK KFYRQRAHAN PFSDHSLTYP KSPADMDWAS 70 80 90 100 110 120 LYPAYAVVKR EQKSAGEEES TPLDEEEQRR LKAITKNVEI ADIGCGFGGL LFALAPKFPD 130 140 150 160 170 180 TLMLEYVQEK VRALRLQNAS IQLYQNASCL RANTMKFLPN FFSKAQLSKI FLCFPDPHFK 190 200 210 220 230 240 QRKHKARIVS YTLNSEYAYV LRPGGVVYTI TDVKDLHEWM VGHFEKHPSF ERCEKEFEEE 250 260 270 280 GEAMDEGVGI MRTETEEGKK VSRNGGMKYV ACFRRVEDPE WP

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445359 Genomic DNA. Translation: EAT77630.2.
RefSeq	XP_001805250.1. XM_001805198.1.
3D structure databases
ProteinModelPortal	Q0TZT0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_15087; SNOT_15087; SNOG_15087.
GeneID	5982178.
KEGG	pno:SNOG_15087.
Phylogenomic databases
KO	K03439.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PHANO

Accession

Primary (citable) accession number: Q0TZT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	February 5, 2008
Last modified:	October 3, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program