Reviewed, UniProtKB/Swiss-Prot Q0TXL7 (PMIP_PHANO)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	SNOG_15771

Organism

Phaeosphaeria nodorum (Glume blotch fungus) (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 790	761	Mitochondrial intermediate peptidase		PRO_0000338591
Sites
	Active site	571	1	By similarity
	Metal binding	570	1	Zinc; catalytic By similarity
	Metal binding	574	1	Zinc; catalytic By similarity
	Metal binding	577	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TXL7-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 3E8AFBC2E8963A45	FASTA	790	89,067
	10 20 30 40 50 60 MLKRLARNNS SPWICSRCLQ QSQRQRRFNS TFAATATARD HAPSALYGLS ASGKTDDDAL 70 80 90 100 110 120 RKVFDNASFW ESFKRGTSNK KPSGIIGNKY LTHPDGFIDF VTVTIQRCNG VVEKVSRAET 130 140 150 160 170 180 IEDFKYMVKD LDKLSDLLCR VIDLADFVRS THPNRQFQIM AVKAYHTVFQ YMNQLNTTPV 190 200 210 220 230 240 LYDQLKKASD IPEVFESWTE EERIVARILM EDFARFGIGL DDATRQKLVD LSGEIAEVGS 250 260 270 280 290 300 QFVEGMSPET PTLKFESKRL KGLDPNLAKA LTKWGETRIS TMHHEAQAVL RFVDDAEVRR 310 320 330 340 350 360 ETYSAVRTAG SSTIARLEKM LKLRAELAQL SGYETFSHMT LENKMAKTPE AVNTFLKALY 370 380 390 400 410 420 EDSRPSVLAD LHELMELKRG DAHQDNFPNR MNAWDKFYYT QKMLSTMEGA YKQRTADSLS 430 440 450 460 470 480 AYFSVGTVLQ GISRLFDRLY GVRLVPQETQ PGEVWEDGVR RLDVISDTEG HIAVLYCDLF 490 500 510 520 530 540 SRPGKTPNPA HFTLRCSREI LPAELEEMQH MPHRFSSPIE AATDGMSVSY NASRNSYFQL 550 560 570 580 590 600 PTIALICDFS KPSSPRPTLL NIHDVRTLFH EMGHALHSIL GRTALQNVSG TRCATDIAEL 610 620 630 640 650 660 PSVLMEHFAF DPSVLALYAR HWDTNAPLPL ALLENRLAID NRNGYSELES QILLAMLDQA 670 680 690 700 710 720 YHSNLPLDPA FNSTSVYHNT YTRFASVPEP AGTRWQGFFG HLFGYGATYY SYLFDRAIAS 730 740 750 760 770 780 RIWKGVFNEG RDGGSLDREK GELYKNEVLR WGGGRDGWVC LAGVLRDGKV GEGGEGAMRE 790 VGKWGIDAGK

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445364 Genomic DNA. Translation: EAT76866.2.
RefSeq	XP_001805910.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	5982840.
Phylogenomic databases
OrthoDB	EOG9DZ3BN.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_PHANO

Accession

Primary (citable) accession number: Q0TXL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 5, 2008
Last modified:	December 15, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)