Reviewed, UniProtKB/Swiss-Prot Q0TVF9 (ALG3_PHANO)

Names and origin

Protein names

Recommended name:
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    EC=2.4.1.130
Alternative name(s):
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Asparagine-linked glycosylation protein 6

Gene names

Name:	ALG3
ORF Names:	SNOG_16505

Organism

Phaeosphaeria nodorum (Glume blotch fungus) (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Transfers an alpha-D-mannosyl residue from dolichyl-phosphate D-mannose into membrane lipid-linked oligosaccharide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase		PRO_0000350932
Regions
	Topological domain	1 – 388	388	Lumenal Potential
	Transmembrane	389 – 409	21	Potential
	Topological domain	410 – 430	21	Cytoplasmic Potential
	Transmembrane	431 – 451	21	Potential
	Topological domain	452 – 467	16	Lumenal Potential
	Transmembrane	468 – 488	21	Potential
	Topological domain	489 – 549	61	Cytoplasmic Potential
	Transmembrane	550 – 570	21	Potential
	Topological domain	571 – 609	39	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0TVF9-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 9077801D747BB97D	FASTA	609	68,416
	10 20 30 40 50 60 MSKLSPALKQ LINAAHSRPG PVPAPPRIQA VYQRIQEEAT ERKLGRPSWL GISTAATMTM 70 80 90 100 110 120 NSPESMIALY NSTSASRPEN ESVQIAEFMR EIGLKCIGFN GIPRTINMLN AFRASLPPTI 130 140 150 160 170 180 ASSLNTTPTR SPSPQNILDT NTRGRALWDA IYRPLETKLI DKLGDAHPDL PVFIINQEYG 190 200 210 220 230 240 GLFTDPPGKP GAKVGRVTTS LVAITCLRAQ QGVGPQVLSH VFGLRKGWED GTWKEEPEAG 250 260 270 280 290 300 SEEAIRWLVS DEGCTWVLEK VDELVEALGG GAGTLSPAID EKPKELTTTK TMSLINRVRD 310 320 330 340 350 360 LATNDEHTRW MIPLLLVVDA ALCGVVIEKI PYTEIDWTTY MQHIALIIKG ERDYTKITGS 370 380 390 400 410 420 TGPLVYPGAH VWIYKQLFKI TDEGRDIQRA QYIFALVYLG TLALVFQCYR KARVPPYVFP 430 440 450 460 470 480 LLILSKRLHS IFLLRCFNDC FAVLGLFAAL FCYQRDQWHV GSFLFATGLN VKMSLLLPLP 490 500 510 520 530 540 AMGVLMIMKL GSREAMTHAM IIFQTTVLFG YPFRKAAFSY FGRAFELSRQ FTYKWTVNWR 550 560 570 580 590 600 FVSEETFLSK PFALGLLSVH VTLLITFFLT RWIKPSKRTP KQFLKIIMPQ AEPRDQDTMA LRITPNLHT

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH445373 Genomic DNA. Translation: EAT76110.2.
RefSeq	XP_001806617.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5983549.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_PHANO

Accession

Primary (citable) accession number: Q0TVF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	February 5, 2008
Last modified:	October 13, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)