Q0TUZ2 (IOLD_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
Short name=THcHDO hydrolase
EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	CPF_0084

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity. HAMAP MF_01669
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01669 Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352540
Regions
	Region	438 – 518	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	489	1	Magnesium By similarity
	Metal binding	516	1	Magnesium By similarity
	Binding site	62	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TUZ2 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 25DDB6E3340B32AD	FASTA	639	70,467
	10 20 30 40 50 60 MRMTTGQALV KFLDNQYVSF DGKEEKFVDG IFTIFGHGIV VGLGEALYEN PGELKVYQGR 70 80 90 100 110 120 NEQGMAHVST AFAKQNNRRK IIACSSSVGP GAANMVTAAA TATVNNIPLL LLPGDSFATR 130 140 150 160 170 180 QPDPVLQQIE QSYNLGITTN DAFKPVCKYW DRINRPEQLM SAMINAMRVL TDPAETGAVC 190 200 210 220 230 240 IALPQDVQGE AYDFPEYFFK KRVHRITRPL AVQEEFEEAL DIIMNKKKPI IICGGGVRYS 250 260 270 280 290 300 EAGEALVDFA EEFNIPICET QAGKSAIKSS HPLNLGGIGV TGNLAANMIA KDADLVIGVG 310 320 330 340 350 360 TRFSDFTTSS KSLFKNPEVD FITVNVSKFH GEKMDAHKII GDAKVCIEEL QAMLEANNYE 370 380 390 400 410 420 SSYEDEIVNA KKAWKEEMKR LTNIKYDENF EALIKPKREG CIEEFSVLTG GLITQTAALG 430 440 450 460 470 480 VIRETIDDDA IVVGAAGSLP GDLQRMWETD VRDSYHMEYG YSCMGYEIAA TLGAKLAEPE 490 500 510 520 530 540 REVYSMVGDG SYLMLHSEMV TAMQEQKKIN ILLFDNCGFG CINNLQMSNG IGSLATEFRY 550 560 570 580 590 600 RDENGKLEGG LIPIDFAKVA SGYGLKTYSV KTLAQLKEAL EDAKKQKVST LIDIKVLPKT 610 620 630 MTDGYDAWWH VGIAGESKID GVNKAFENKE KNLKAARRY

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG84674.1.
RefSeq	YP_694548.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TUZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TUZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4201952.
GenomeReviews	Gene locus CPF_0084 in contig CP000246_GR.
KEGG	cpf:CPF_0084.
PATRIC	19482469. VBICloPer106549_0073.
TIGR	CPF_0084.
Phylogenomic databases
eggNOG	COG3962.
HOGENOM	HBG294332.
OMA	GEAYDFP.
ProtClustDB	CLSK2303116.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_0084-MONOMER.
Family and domain databases
HAMAP	MF_01669. IolD. [Tree]
InterPro	IPR023757. THcHDO_hydrolase. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K03336.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_CLOP1

Accession

Primary (citable) accession number: Q0TUZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program