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Q0TUR6 (BGAL_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase Pbg

Short name=Beta-gal
EC=3.2.1.23
Gene names
Name:pbg
Ordered Locus Names:CPF_0160
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Sequence caution

The sequence BAA08485.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Beta-galactosidase Pbg
PRO_0000407688

Regions

Region366 – 3694Substrate binding

Sites

Active site1571Proton donor By similarity
Active site3181Nucleophile By similarity
Metal binding1221Zinc By similarity
Metal binding1621Zinc By similarity
Metal binding1641Zinc By similarity
Metal binding1671Zinc By similarity
Binding site1181Substrate By similarity
Binding site1561Substrate By similarity
Binding site3261Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TUR6 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 6034BFC444243857

FASTA68979,981
        10         20         30         40         50         60 
MKNYGPISSK VTKMLHGADY NPEQWIDMPN IWGEDVRLMK LSHTNVVAVG IFSWTMLEPE 

        70         80         90        100        110        120 
EGKFNFEWLD EIMDLMHKNG NYVILATPSG AKPIWMAHKY PETLRVAQNR VRNLYGERHN 

       130        140        150        160        170        180 
HCYTSPIYRE KIAIIDRLLA ERYKDHPALI LWHISNEFEG QCYCPLCEEA FRDFLREKYD 

       190        200        210        220        230        240 
NDINKLNKAW WTKFWSHTYA SFDEIEAPAP HGEPALHGLN LDWMRFVTHQ TLDYYKHERS 

       250        260        270        280        290        300 
ILKEITPDIP VTTNFHDYIS LFRGIDYWKF APYLDVVSWD NYPYWHGERT DDHEGSRIGF 

       310        320        330        340        350        360 
VHDLNRAILN GKPFMMMESS PSSTNWQPVA KLRRPGMHVL SSLQAVAHGS DTVQYFQWRK 

       370        380        390        400        410        420 
SRGSSEKFHG AVVDHCGHEN TRVFRDVTKV GEILSKLDDV IGTSVEPQVA VIYDWENYWA 

       430        440        450        460        470        480 
INDAQGPRIE QKDYFETCQK HYKAFWDMSI PTDVINMDCD FSKYKVVVAP MLYMVRPGVG 

       490        500        510        520        530        540 
ERLEEFVKNG GTLVTTYWSG IVDENDLCFL GGFPGPLKKV TGIWAEELDA LYDEDVNYVS 

       550        560        570        580        590        600 
VEEGNSLGMK GEYEARIFCD LIHSEGAEVL ATYKTDFYKG MPALTCNNFG EGQAYYIAFR 

       610        620        630        640        650        660 
NNDEFLSDFY SSLAKKLTLK RAIEIDLPKG INAQVRMDEK NEFVFFMNFS SEEKTIDIKD 

       670        680 
LDLTDMVTGE KVAKEMEIEP YGVRIVRRK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of flanking regions of the pfoA gene of Clostridium perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking region."
Shimizu T., Kobayashi T., Ba-Thein W., Ohtani K., Hayashi H.
Microbiol. Immunol. 39:677-686(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13124 / NCTC 8237 / Type A.
[2]"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens."
Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. expand/collapse author list , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T.
Genome Res. 16:1031-1040(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D49537 Genomic DNA. Translation: BAA08485.1. Different initiation.
CP000246 Genomic DNA. Translation: ABG83924.1.
RefSeqYP_694624.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TUR6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195103.CPF_0160.

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG83924; ABG83924; CPF_0160.
GeneID4202873.
KEGGcpf:CPF_0160.
PATRIC19482617. VBICloPer106549_0147.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000117811.
KOK12308.
OMACFLGGFP.
OrthoDBEOG6GTZGG.

Enzyme and pathway databases

BioCycCPER195103:GHAW-169-MONOMER.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL_CLOP1
AccessionPrimary (citable) accession number: Q0TUR6
Secondary accession number(s): Q46253, Q59312
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: September 5, 2006
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries