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Protein

Beta-galactosidase Pbg

Gene

pbg

Organism
Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181SubstrateBy similarity
Metal bindingi122 – 1221ZincBy similarity
Binding sitei156 – 1561SubstrateBy similarity
Active sitei157 – 1571Proton donorBy similarity
Metal bindingi162 – 1621ZincBy similarity
Metal bindingi164 – 1641ZincBy similarity
Metal bindingi167 – 1671ZincBy similarity
Active sitei318 – 3181NucleophileBy similarity
Binding sitei326 – 3261SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCPER195103:GHAW-169-MONOMER.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase Pbg (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:pbgImported
Ordered Locus Names:CPF_0160
OrganismiClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001823 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689Beta-galactosidase PbgPRO_0000407688Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi195103.CPF_0160.

Structurei

3D structure databases

ProteinModelPortaliQ0TUR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni366 – 3694Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000117811.
KOiK12308.
OMAiRFVTHQT.
OrthoDBiEOG6GTZGG.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TUR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNYGPISSK VTKMLHGADY NPEQWIDMPN IWGEDVRLMK LSHTNVVAVG
60 70 80 90 100
IFSWTMLEPE EGKFNFEWLD EIMDLMHKNG NYVILATPSG AKPIWMAHKY
110 120 130 140 150
PETLRVAQNR VRNLYGERHN HCYTSPIYRE KIAIIDRLLA ERYKDHPALI
160 170 180 190 200
LWHISNEFEG QCYCPLCEEA FRDFLREKYD NDINKLNKAW WTKFWSHTYA
210 220 230 240 250
SFDEIEAPAP HGEPALHGLN LDWMRFVTHQ TLDYYKHERS ILKEITPDIP
260 270 280 290 300
VTTNFHDYIS LFRGIDYWKF APYLDVVSWD NYPYWHGERT DDHEGSRIGF
310 320 330 340 350
VHDLNRAILN GKPFMMMESS PSSTNWQPVA KLRRPGMHVL SSLQAVAHGS
360 370 380 390 400
DTVQYFQWRK SRGSSEKFHG AVVDHCGHEN TRVFRDVTKV GEILSKLDDV
410 420 430 440 450
IGTSVEPQVA VIYDWENYWA INDAQGPRIE QKDYFETCQK HYKAFWDMSI
460 470 480 490 500
PTDVINMDCD FSKYKVVVAP MLYMVRPGVG ERLEEFVKNG GTLVTTYWSG
510 520 530 540 550
IVDENDLCFL GGFPGPLKKV TGIWAEELDA LYDEDVNYVS VEEGNSLGMK
560 570 580 590 600
GEYEARIFCD LIHSEGAEVL ATYKTDFYKG MPALTCNNFG EGQAYYIAFR
610 620 630 640 650
NNDEFLSDFY SSLAKKLTLK RAIEIDLPKG INAQVRMDEK NEFVFFMNFS
660 670 680
SEEKTIDIKD LDLTDMVTGE KVAKEMEIEP YGVRIVRRK
Length:689
Mass (Da):79,981
Last modified:September 5, 2006 - v1
Checksum:i6034BFC444243857
GO

Sequence cautioni

The sequence BAA08485.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49537 Genomic DNA. Translation: BAA08485.1. Different initiation.
CP000246 Genomic DNA. Translation: ABG83924.1.
RefSeqiYP_694624.1. NC_008261.1.

Genome annotation databases

EnsemblBacteriaiABG83924; ABG83924; CPF_0160.
KEGGicpf:CPF_0160.
PATRICi19482617. VBICloPer106549_0147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49537 Genomic DNA. Translation: BAA08485.1. Different initiation.
CP000246 Genomic DNA. Translation: ABG83924.1.
RefSeqiYP_694624.1. NC_008261.1.

3D structure databases

ProteinModelPortaliQ0TUR6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi195103.CPF_0160.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG83924; ABG83924; CPF_0160.
KEGGicpf:CPF_0160.
PATRICi19482617. VBICloPer106549_0147.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000117811.
KOiK12308.
OMAiRFVTHQT.
OrthoDBiEOG6GTZGG.

Enzyme and pathway databases

BioCyciCPER195103:GHAW-169-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of flanking regions of the pfoA gene of Clostridium perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking region."
    Shimizu T., Kobayashi T., Ba-Thein W., Ohtani K., Hayashi H.
    Microbiol. Immunol. 39:677-686(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13124 / NCTC 8237 / Type AImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13124 / NCTC 8237 / Type AImported.

Entry informationi

Entry nameiBGAL_CLOP1
AccessioniPrimary (citable) accession number: Q0TUR6
Secondary accession number(s): Q46253, Q59312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: September 5, 2006
Last modified: April 29, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.