Q0TUR6 (BGAL_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase Pbg
Short name=Beta-gal
EC=3.2.1.23

Gene names

Name:	pbg
Ordered Locus Names:	CPF_0160

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 42 family.
Sequence caution	The sequence BAA08485.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Beta-galactosidase Pbg		PRO_0000407688
Regions
	Region	366 – 369	4	Substrate binding
Sites
	Active site	157	1	Proton donor By similarity
	Active site	318	1	Nucleophile By similarity
	Metal binding	122	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Metal binding	164	1	Zinc By similarity
	Metal binding	167	1	Zinc By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	326	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TUR6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6034BFC444243857	FASTA	689	79,981
	10 20 30 40 50 60 MKNYGPISSK VTKMLHGADY NPEQWIDMPN IWGEDVRLMK LSHTNVVAVG IFSWTMLEPE 70 80 90 100 110 120 EGKFNFEWLD EIMDLMHKNG NYVILATPSG AKPIWMAHKY PETLRVAQNR VRNLYGERHN 130 140 150 160 170 180 HCYTSPIYRE KIAIIDRLLA ERYKDHPALI LWHISNEFEG QCYCPLCEEA FRDFLREKYD 190 200 210 220 230 240 NDINKLNKAW WTKFWSHTYA SFDEIEAPAP HGEPALHGLN LDWMRFVTHQ TLDYYKHERS 250 260 270 280 290 300 ILKEITPDIP VTTNFHDYIS LFRGIDYWKF APYLDVVSWD NYPYWHGERT DDHEGSRIGF 310 320 330 340 350 360 VHDLNRAILN GKPFMMMESS PSSTNWQPVA KLRRPGMHVL SSLQAVAHGS DTVQYFQWRK 370 380 390 400 410 420 SRGSSEKFHG AVVDHCGHEN TRVFRDVTKV GEILSKLDDV IGTSVEPQVA VIYDWENYWA 430 440 450 460 470 480 INDAQGPRIE QKDYFETCQK HYKAFWDMSI PTDVINMDCD FSKYKVVVAP MLYMVRPGVG 490 500 510 520 530 540 ERLEEFVKNG GTLVTTYWSG IVDENDLCFL GGFPGPLKKV TGIWAEELDA LYDEDVNYVS 550 560 570 580 590 600 VEEGNSLGMK GEYEARIFCD LIHSEGAEVL ATYKTDFYKG MPALTCNNFG EGQAYYIAFR 610 620 630 640 650 660 NNDEFLSDFY SSLAKKLTLK RAIEIDLPKG INAQVRMDEK NEFVFFMNFS SEEKTIDIKD 670 680 LDLTDMVTGE KVAKEMEIEP YGVRIVRRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of flanking regions of the pfoA gene of Clostridium perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking region." Shimizu T., Kobayashi T., Ba-Thein W., Ohtani K., Hayashi H. Microbiol. Immunol. 39:677-686(1995) [PubMed: 8577281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.
[2]	"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49537 Genomic DNA. Translation: BAA08485.1. Different initiation. CP000246 Genomic DNA. Translation: ABG83924.1.
RefSeq	YP_694624.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TUR6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TUR6.
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4202873.
GenomeReviews	Gene locus CPF_0160 in contig CP000246_GR.
KEGG	cpf:CPF_0160.
PATRIC	19482617. VBICloPer106549_0147.
TIGR	CPF_0160.
Phylogenomic databases
eggNOG	COG1874.
HOGENOM	HBG476453.
OMA	HGSDTVQ.
ProtClustDB	CLSK810799.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_0160-MONOMER.
Family and domain databases
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR013780. Glyco_hydro_13_b. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K12308.
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGAL_CLOP1

Accession

Primary (citable) accession number: Q0TUR6
Secondary accession number(s): Q46253, Q59312

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program