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Q0TU06 (TRMB_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CPF_0427
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

tRNA methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288140

Sites

Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site1021S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1611Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TU06 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 2D65C62609EE6C48

FASTA21925,984
        10         20         30         40         50         60 
MRMRRKPWAR PELEACDFFV ANPKENKGNW KNTFKNTENP IYLELGCGKG TFMAVHGSDH 

        70         80         90        100        110        120 
PNINYIAIDI KDEVLVLAKR NIEKAYEEKN KALDNVKLMP QEIALIDTIL DSNDKIERIY 

       130        140        150        160        170        180 
INFCNPWPKD RHKKRRLTHT RQLTKYRDFL VDGGEIHFKT DDDELFEESL EYFKECNFEI 

       190        200        210 
TYITRDLHNS GYEYNVVTEH EEMFSKQGIK IKFLIAKKL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000246 Genomic DNA. Translation: ABG83472.1.
RefSeqYP_694884.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TU06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195103.CPF_0427.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG83472; ABG83472; CPF_0427.
GeneID4203736.
KEGGcpf:CPF_0427.
PATRIC19483156. VBICloPer106549_0406.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCPER195103:GHAW-442-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CLOP1
AccessionPrimary (citable) accession number: Q0TU06
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 5, 2006
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways