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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195103:GHAW-675-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CPF_0657
OrganismiClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Leucine--tRNA ligasePRO_1000009329Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi195103.CPF_0657.

Structurei

3D structure databases

ProteinModelPortaliQ0TTD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TTD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNYSTAIDK KWQEKWAESG LYKFDPNKEG EKLYVLEMFS YPSGSQLHAG
60 70 80 90 100
HWFNYGPVDS WARFKRMQGY NVFQPMGFDA FGLPAENFAI KTGIHPQDST
110 120 130 140 150
IKNIAKMEEQ LKAMGAMFNW ENEVVTCSPE YYKWTQWLFL KLYEKGLAYR
160 170 180 190 200
KKAPVNWCPS CQTVLANEQV VDGACERCST EVTKKDLTQW FFKITDYADE
210 220 230 240 250
LLDKLDDLDW PEKTVSMQKH WIGRSTGSQV NFKVKDSDLN FDVFTTRVDT
260 270 280 290 300
LCGVSYVVLA PENPLVDEIV SAEQKEAVEN YKEEAKKQSD IERQSISREK
310 320 330 340 350
TGVFTGAYAI HPLTGKEVPI WVGDYVLATY GTGAVMAVPA HDERDFAFAE
360 370 380 390 400
KFNLPINRVI EAKDGSETNL PFCEHGILVN SGEFDGLTTD EAKEKIVEKL
410 420 430 440 450
ASMGLGEKKV NFRLRDWLVS RQRYWGAPIP VVYCEECGIV PVPESQLPVE
460 470 480 490 500
LPYDVEFAPD GKSPLAKSEA FVNTTCPHCG KPAKRETDTL DTFVCSSWYY
510 520 530 540 550
LRYPDNKNTE APFNPELINK MLPVDKYVGG PEHACMHLLY ARFITKALRD
560 570 580 590 600
MGYLNFDEPF TSLTHQGLIL GPDGLKMSKS KGNTISPDDY IKEYGADVFR
610 620 630 640 650
MYLMFGFAYT EGGAWSDDGI KSVNRFVERI ERIIDTAREA ISKGENNKTT
660 670 680 690 700
MDKAEKELNY WRHNTIKSVT DDTDKLQFNT AIARMMEFIN ALSKYTQEKE
710 720 730 740 750
MNLDFLKDVV SDYLRLLAPF APHFSEEQWS LLGNSYSIFN EAWPKFDPKA
760 770 780 790 800
LVKDEVEIAI QVNGKIKNKI MVSSDLDEEG IKAAALADEK IIASTEGKTV
810
VKVIVIKGRL VNIVVK
Length:816
Mass (Da):92,693
Last modified:September 5, 2006 - v1
Checksum:i2A5FDC10D11E5289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA. Translation: ABG83404.1.
RefSeqiWP_003457020.1. NC_008261.1.
YP_695110.1. NC_008261.1.

Genome annotation databases

EnsemblBacteriaiABG83404; ABG83404; CPF_0657.
KEGGicpf:CPF_0657.
PATRICi19483620. VBICloPer106549_0633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA. Translation: ABG83404.1.
RefSeqiWP_003457020.1. NC_008261.1.
YP_695110.1. NC_008261.1.

3D structure databases

ProteinModelPortaliQ0TTD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi195103.CPF_0657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG83404; ABG83404; CPF_0657.
KEGGicpf:CPF_0657.
PATRICi19483620. VBICloPer106549_0633.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCPER195103:GHAW-675-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13124 / NCTC 8237 / Type A.

Entry informationi

Entry nameiSYL_CLOP1
AccessioniPrimary (citable) accession number: Q0TTD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.