Q0TTB4 (PUR7_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	CPF_0673

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137

PRO_1000018692

Secondary structure

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235

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Beta strand

14 – 21

8

Beta strand

24 – 29

6

Beta strand

31 – 35

5

Beta strand

40 – 43

4

Helix

50 – 64

15

Beta strand

69 – 74

6

Beta strand

76 – 83

8

Beta strand

89 – 97

9

Helix

102 – 105

4

Beta strand

113 – 123

11

Helix

135 – 138

4

Helix

146 – 168

23

Turn

169 – 171

3

Beta strand

172 – 180

9

Beta strand

190 – 192

3

Turn

196 – 198

3

Beta strand

199 – 204

6

Helix

213 – 216

4

Helix

227 – 233

7

Sequences

	Sequence		Length	Mass (Da)
	Q0TTB4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 3A41A843F0D61F96	FASTA	235	27,003
	10 20 30 40 50 60 MVNQLEMLYE GKAKKIYATD KEDMVIVHYK DDATAFNGEK KAQIESKGVL NNEITSLIFE 70 80 90 100 110 120 MLNKEGIKTH FVEKLNDRDQ LCKKVEIVPL EVIVRNVAAG SMAKRLGLEE GYELKTTVFE 130 140 150 160 170 180 LSYKDDSLGD PLINDYHAVG IGATTFEELN KIYEITAKVN EILKEAFKKQ NINLIDFKLE 190 200 210 220 230 FGRYNGEILL ADEISPDTCR FWDATTGEKM DKDRFRRDMG NVINGYREVL NRLRN

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000246 Genomic DNA. Translation: ABG83396.1.

RefSeq

YP_695126.1. NC_008261.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NUA	X-ray	1.40	A/B	1-235	[»]

ProteinModelPortal

Q0TTB4.

SMR

Q0TTB4. Positions 1-234.

ModBase

Search...

Protein-protein interaction databases

STRING

Q0TTB4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

4203660.

GenomeReviews

Gene locus CPF_0673 in contig CP000246_GR.

KEGG

cpf:CPF_0673.

PATRIC

19483650. VBICloPer106549_0648.

TIGR

CPF_0673.

Phylogenomic databases

eggNOG

COG0152.

HOGENOM

HBG306070.

OMA

YKDDALG.

ProtClustDB

PRK09362.

Enzyme and pathway databases

BioCyc

CPER195103:CPF_0673-MONOMER.

Family and domain databases

HAMAP

MF_00137. SAICAR_synth.
[Tree]

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

KO

K01923.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. PurC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PUR7_CLOP1

Accession

Primary (citable) accession number: Q0TTB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program