Q0TTB2 (PUR5_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	CPF_0675

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_0000258347

Sequences

	Sequence		Length	Mass (Da)
	Q0TTB2 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 78B8A6FCA8B44275	FASTA	333	36,440
	10 20 30 40 50 60 MLTYKEAGVN IEEGYRSVKL IKEYAKKTMS EYVLNGLGSF AGMVELPEGY KKPVLVSGTD 70 80 90 100 110 120 GVGTKLDIAC KKRKFDTVGI DCVAMCVNDI LCHGAKPLFF LDYIACGKLE AEVSSDLVKG 130 140 150 160 170 180 VAEGCIESQC SLIGGETAEM PGMYKEGDYD IAGFAVGIVD KDKIINGKDI KSGDKLIGIA 190 200 210 220 230 240 SSGVHSNGYS LIRKVFKNLD EDFNGKAIWE ELLTPTKIYV KPVLSLLEKF NIKGMAHVTG 250 260 270 280 290 300 GGFYENLPRM LSKEGLSIVI NKNSYEIPEI FKKLMELGVK EEEMYNTFNM GIGFVLCVEE 310 320 330 DEVEEVLKEL SKQGEKAFEI GYINAGGEGV CIK

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG84389.1.
RefSeq	YP_695128.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TTB2.
SMR	Q0TTB2. Positions 16-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TTB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4201607.
GenomeReviews	Gene locus CPF_0675 in contig CP000246_GR.
KEGG	cpf:CPF_0675.
PATRIC	19483654. VBICloPer106549_0650.
TIGR	CPF_0675.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HBG531222.
OMA	PENIPRV.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_0675-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_CLOP1

Accession

Primary (citable) accession number: Q0TTB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program