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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei296 – 2961ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195103:GHAW-1273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CPF_1255
OrganismiClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001823 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552Glutamate--tRNA ligasePRO_0000367650Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi195103.CPF_1255.

Structurei

3D structure databases

ProteinModelPortaliQ0TRN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi293 – 2975"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNKLTWIN.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TRN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYENLANLI FPNIDKTPEY YFEKYPKRDL KEGAKVLRYA PSPTGFQHIG
60 70 80 90 100
GVFASLINER LAHQSGGIFY LRIEDTDQKR EVEGAIDDTI KTMHNFGMDF
110 120 130 140 150
DEGITGENSE KGAYAPYKQS QRADIYRAFV KDLLRKGLAY PCFMTSEELE
160 170 180 190 200
ALREKQIAEK LTPGCYGEFA KYRDLSPEEA IKRIEAGESY VIRMKSPGNP
210 220 230 240 250
EKRVVAHDMI KGEVSFPENL QDVVIIKGDG LPTYHFAHAI DDTLMRTTHV
260 270 280 290 300
IRGEEWLSSL PIHLQMFEVL GVEAPKYAHI PTIMKMDGSS KRKLSKRKDP
310 320 330 340 350
ESAVSYYSEK GYPSQSVIEY LLNIINSAFE EWRAENPDAD YHDYKVELDK
360 370 380 390 400
MSKSGALFDL VKLNDVSKDV ICKMKPEVVY DLYTNWAKEY DKEMYDLVTS
410 420 430 440 450
KEAMMKEVFN IDKEGPKPRK DFAKWDEVRE KIFYFFDELF DKETANDVEL
460 470 480 490 500
PKTLELEEAK RIIEAYEKAY NFNTDKDTWF SDLKEVAVEL GYATDRKKYK
510 520 530 540 550
KNPEEYKGMV SDVAGAVRAA LTHRANTPDL YTIMQIMGEE AVRERFKKFL

SL
Length:552
Mass (Da):63,749
Last modified:September 4, 2006 - v1
Checksum:i47010E5CC99C8C52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA. Translation: ABG82688.1.
RefSeqiYP_695701.1. NC_008261.1.

Genome annotation databases

EnsemblBacteriaiABG82688; ABG82688; CPF_1255.
KEGGicpf:CPF_1255.
PATRICi19484782. VBICloPer106549_1211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA. Translation: ABG82688.1.
RefSeqiYP_695701.1. NC_008261.1.

3D structure databases

ProteinModelPortaliQ0TRN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi195103.CPF_1255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG82688; ABG82688; CPF_1255.
KEGGicpf:CPF_1255.
PATRICi19484782. VBICloPer106549_1211.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNKLTWIN.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciCPER195103:GHAW-1273-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13124 / NCTC 8237 / Type A.

Entry informationi

Entry nameiSYE_CLOP1
AccessioniPrimary (citable) accession number: Q0TRN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: September 4, 2006
Last modified: March 31, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.