Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei296ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CPF_1255
OrganismiClostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001823 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676501 – 552Glutamate--tRNA ligaseAdd BLAST552

Proteomic databases

PRIDEiQ0TRN9

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi195103.CPF_1255

Structurei

3D structure databases

ProteinModelPortaliQ0TRN9
SMRiQ0TRN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 51"HIGH" regionUniRule annotationAdd BLAST11
Motifi293 – 297"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiRNIEGKD
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Q0TRN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYENLANLI FPNIDKTPEY YFEKYPKRDL KEGAKVLRYA PSPTGFQHIG
60 70 80 90 100
GVFASLINER LAHQSGGIFY LRIEDTDQKR EVEGAIDDTI KTMHNFGMDF
110 120 130 140 150
DEGITGENSE KGAYAPYKQS QRADIYRAFV KDLLRKGLAY PCFMTSEELE
160 170 180 190 200
ALREKQIAEK LTPGCYGEFA KYRDLSPEEA IKRIEAGESY VIRMKSPGNP
210 220 230 240 250
EKRVVAHDMI KGEVSFPENL QDVVIIKGDG LPTYHFAHAI DDTLMRTTHV
260 270 280 290 300
IRGEEWLSSL PIHLQMFEVL GVEAPKYAHI PTIMKMDGSS KRKLSKRKDP
310 320 330 340 350
ESAVSYYSEK GYPSQSVIEY LLNIINSAFE EWRAENPDAD YHDYKVELDK
360 370 380 390 400
MSKSGALFDL VKLNDVSKDV ICKMKPEVVY DLYTNWAKEY DKEMYDLVTS
410 420 430 440 450
KEAMMKEVFN IDKEGPKPRK DFAKWDEVRE KIFYFFDELF DKETANDVEL
460 470 480 490 500
PKTLELEEAK RIIEAYEKAY NFNTDKDTWF SDLKEVAVEL GYATDRKKYK
510 520 530 540 550
KNPEEYKGMV SDVAGAVRAA LTHRANTPDL YTIMQIMGEE AVRERFKKFL

SL
Length:552
Mass (Da):63,749
Last modified:September 5, 2006 - v1
Checksum:i47010E5CC99C8C52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA Translation: ABG82688.1
RefSeqiWP_011590635.1, NC_008261.1

Genome annotation databases

EnsemblBacteriaiABG82688; ABG82688; CPF_1255
GeneIDi29571633
KEGGicpf:CPF_1255

Similar proteinsi

Entry informationi

Entry nameiSYE_CLOP1
AccessioniPrimary (citable) accession number: Q0TRN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health