Q0TRK5 (COBT_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	CPF_1289

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). HAMAP MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP MF_00230		PRO_1000021585
Sites
	Active site	315	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TRK5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 64EC972E80C57CA3	FASTA	361	38,853
	10 20 30 40 50 60 MLNEVLKGIE GLDNDMILKA KNRVDSLAKP LGSLGKLEDI AIRLSGITGN MFNNIDKKCV 70 80 90 100 110 120 IIMSSDNGVE EEGVASAPQC VTLLQTKNFI KGTTGVATLA KSNGTDLMVF DVGINSDEVV 130 140 150 160 170 180 EGVINRKISK GTKNIYKEPA MTYEEAKKSL EIGIEAVKIA KEKGYKILGV GEMGIGNTTT 190 200 210 220 230 240 SAAVLKALIG CETSQVVGKG GGINNASFEK KKRIVEEVVK KHNINFDDPI DIISKVGGYD 250 260 270 280 290 300 IGAMTGVFLG AAFYRIPVVI DGFISVVAAL LANRLNPLVK EFCFTSHKSQ EIGYELAIKE 310 320 330 340 350 360 LGLDPMLDLN MRLGEGSGCP IAFSVIDFAT AMMNNMATFE EGNIDNSYLE DVKDEECYIV L

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG83123.1.
RefSeq	YP_695735.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TRK5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TRK5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4202333.
GenomeReviews	Gene locus CPF_1289 in contig CP000246_GR.
KEGG	cpf:CPF_1289.
PATRIC	19484850. VBICloPer106549_1245.
TIGR	CPF_1289.
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HBG634399.
OMA	FICSVAA.
ProtClustDB	PRK00105.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_1289-MONOMER.
Family and domain databases
HAMAP	MF_00230. CobT. [Tree]
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Gene3D	G3DSA:1.10.1610.10. Nict_dMeBzImd_PRibTrfase_N. 1 hit.
KO	K00768.
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. CobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_CLOP1

Accession

Primary (citable) accession number: Q0TRK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program