ID   MIAA_CLOP1              Reviewed;         310 AA.
AC   Q0TRD4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=tRNA Delta(2)-isopentenylpyrophosphate transferase;
DE            Short=IPP transferase;
DE            EC=2.5.1.8;
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE            Short=IPTase;
DE            Short=IPPT;
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE            Short=DMAPP:tRNA dimethylallyltransferase;
DE            Short=DMATase;
GN   Name=miaA; OrderedLocusNames=CPF_1360;
OS   Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195103;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R.,
RA   DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C.,
RA   Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J.,
RA   Sullivan S.A., Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S.,
RA   Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H.,
RA   Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I.,
RA   Melville S.B., Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial
RT   pathogen, Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate +
CC       tRNA containing 6-isopentenyladenosine.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
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DR   EMBL; CP000246; ABG83920.1; -; Genomic_DNA.
DR   RefSeq; YP_695806.1; -.
DR   GeneID; 4202869; -.
DR   GenomeReviews; CP000246_GR; CPF_1360.
DR   KEGG; cpf:CPF_1360; -.
DR   TIGR; CPF_1360; -.
DR   HOGENOM; Q0TRD4; -.
DR   OMA; Q0TRD4; VNADSMQ.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004811; F:tRNA isopentenyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR   HAMAP; MF_00185; -; 1.
DR   InterPro; IPR002627; IPPT.
DR   InterPro; IPR018022; tRNA_delta_PyrP_Trfase.
DR   PANTHER; PTHR11088; IPPT; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   ProDom; PD004674; IPPT; 1.
DR   ProDom; PD005388; IPPtrans_like; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Magnesium; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; tRNA processing.
FT   CHAIN         1    310       tRNA Delta(2)-isopentenylpyrophosphate
FT                                transferase.
FT                                /FTId=PRO_1000020588.
FT   NP_BIND      10     17       ATP (Potential).
FT   REGION       12     17       Substrate binding (By similarity).
FT   REGION       35     38       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        101    101       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        124    124       Interaction with substrate tRNA (By
FT                                similarity).
SQ   SEQUENCE   310 AA;  35672 MW;  D56BB8B0F14EC0CF CRC64;
     MNNNLLIIAG PTAVGKSDLS VDLAKKLNGE IISVDSMQIY KYMDIGSAKI SKEEMGGVPH
     YLIDFVDPSK EFSVAEFKDL ATEKIKDIQS RGKLPILVGG TGLYINSIIC NMNFAESDKD
     EEYREELEKI ANEHGNEYLH EMLKDIDLES YNSIHFNNRK RVIRALETYK LTGKPFSSFK
     AKNSIYETPY NIYYYVLNMD RAKLYDRINK RVDIMFEKGL LEEVKNLKAM GLTDDMQSMK
     GIGYKEVLYY LDGKISLEQC IEMIKQGSRN YAKRQLTWFR KDPRAIFIDK DTFASEEDIS
     SKIINDIINS
//