Reviewed, UniProtKB/Swiss-Prot Q0TRD4 (MIAA_CLOP1)

Names and origin

Protein names

Recommended name:
    tRNA Delta(2)-isopentenylpyrophosphate transferase
      Short name=IPP transferase
    EC=2.5.1.8
Alternative name(s):
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
      Short name=IPTase
      Short name=IPPT
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
      Short name=DMAPP:tRNA dimethylallyltransferase
      Short name=DMATase

Gene names

Name:	miaA
Ordered Locus Names:	CPF_1360

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity.
Catalytic activity	Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW tRNA isopentenyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	tRNA Delta(2)-isopentenylpyrophosphate transferase		PRO_1000020588
Regions
	Nucleotide binding	10 – 17	8	ATP Potential
	Region	12 – 17	6	Substrate binding By similarity
	Region	35 – 38	4	Interaction with substrate tRNA By similarity
Sites
	Site	101	1	Interaction with substrate tRNA By similarity
	Site	124	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TRD4-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D56BB8B0F14EC0CF	FASTA	310	35,672
	10 20 30 40 50 60 MNNNLLIIAG PTAVGKSDLS VDLAKKLNGE IISVDSMQIY KYMDIGSAKI SKEEMGGVPH 70 80 90 100 110 120 YLIDFVDPSK EFSVAEFKDL ATEKIKDIQS RGKLPILVGG TGLYINSIIC NMNFAESDKD 130 140 150 160 170 180 EEYREELEKI ANEHGNEYLH EMLKDIDLES YNSIHFNNRK RVIRALETYK LTGKPFSSFK 190 200 210 220 230 240 AKNSIYETPY NIYYYVLNMD RAKLYDRINK RVDIMFEKGL LEEVKNLKAM GLTDDMQSMK 250 260 270 280 290 300 GIGYKEVLYY LDGKISLEQC IEMIKQGSRN YAKRQLTWFR KDPRAIFIDK DTFASEEDIS 310 SKIINDIINS

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000246 Genomic DNA. Translation: ABG83920.1.
RefSeq	YP_695806.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4202869.
GenomeReviews	Gene locus CPF_1360 in contig CP000246_GR.
KEGG	cpf:CPF_1360.
TIGR	CPF_1360.
Phylogenomic databases
HOGENOM	Q0TRD4.
OMA	Q0TRD4. VNADSMQ.
Family and domain databases
HAMAP	MF_00185. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
ProDom	PD004674. IPPT. 1 hit. PD005388. IPPtrans_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00174. miaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_CLOP1

Accession

Primary (citable) accession number: Q0TRD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)