Q0TRD4 (MIAA_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA dimethylallyltransferase
EC=2.5.1.75

Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name=DMAPP:tRNA dimethylallyltransferase
Short name=DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name=IPP transferase
Short name=IPPT
Short name=IPTase

Gene names

Name:	miaA
Ordered Locus Names:	CPF_1360

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity. HAMAP MF_00185
Catalytic activity	Dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity. HAMAP MF_00185
Subunit structure	Monomer By similarity. HAMAP MF_00185
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	tRNA dimethylallyltransferase HAMAP MF_00185		PRO_1000020588
Regions
	Nucleotide binding	10 – 17	8	ATP Potential
	Region	12 – 17	6	Substrate binding By similarity
	Region	35 – 38	4	Interaction with substrate tRNA By similarity
Sites
	Site	101	1	Interaction with substrate tRNA By similarity
	Site	124	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TRD4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D56BB8B0F14EC0CF	FASTA	310	35,672
	10 20 30 40 50 60 MNNNLLIIAG PTAVGKSDLS VDLAKKLNGE IISVDSMQIY KYMDIGSAKI SKEEMGGVPH 70 80 90 100 110 120 YLIDFVDPSK EFSVAEFKDL ATEKIKDIQS RGKLPILVGG TGLYINSIIC NMNFAESDKD 130 140 150 160 170 180 EEYREELEKI ANEHGNEYLH EMLKDIDLES YNSIHFNNRK RVIRALETYK LTGKPFSSFK 190 200 210 220 230 240 AKNSIYETPY NIYYYVLNMD RAKLYDRINK RVDIMFEKGL LEEVKNLKAM GLTDDMQSMK 250 260 270 280 290 300 GIGYKEVLYY LDGKISLEQC IEMIKQGSRN YAKRQLTWFR KDPRAIFIDK DTFASEEDIS 310 SKIINDIINS

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG83920.1.
RefSeq	YP_695806.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TRD4.
SMR	Q0TRD4. Positions 5-117.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TRD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4202869.
GenomeReviews	Gene locus CPF_1360 in contig CP000246_GR.
KEGG	cpf:CPF_1360.
PATRIC	19484990. VBICloPer106549_1315.
TIGR	CPF_1360.
Phylogenomic databases
eggNOG	COG0324.
HOGENOM	HBG559459.
OMA	IGYKELY.
ProtClustDB	PRK00091.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_1360-MONOMER.
Family and domain databases
HAMAP	MF_00185. IPP_trans. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
KO	K00791.
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00174. MiaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_CLOP1

Accession

Primary (citable) accession number: Q0TRD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program