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Protein

Enolase

Gene

eno

Organism
Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk), Pyruvate kinase (CPF_1281)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei158SubstrateUniRule annotation1
Binding sitei167SubstrateUniRule annotation1
Active sitei208Proton donorUniRule annotation1
Metal bindingi245MagnesiumUniRule annotation1
Metal bindingi288MagnesiumUniRule annotation1
Binding sitei288SubstrateUniRule annotation1
Metal bindingi315MagnesiumUniRule annotation1
Binding sitei315SubstrateUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei391SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:CPF_1505
OrganismiClostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001823 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002670191 – 431EnolaseAdd BLAST431

Proteomic databases

PRIDEiQ0TQZ2

Interactioni

Protein-protein interaction databases

STRINGi195103.CPF_1505

Structurei

3D structure databases

ProteinModelPortaliQ0TQZ2
SMRiQ0TQZ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni367 – 370Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072173
KOiK01689
OMAiEFMIIPV
OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q0TQZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYIEIIDV VARQILDSRC FPTVEVEVYL EDGTVGRAAV PSGASTGIYE
60 70 80 90 100
AVELRDGDKD KYLGKGVEKA VANVNDTIAE EIIGLNVLDQ AYIDKTLIEL
110 120 130 140 150
DGTKNKGKLG ANAILGVSLA VAQAAANYLG MPLYQYIGGV NAKVLPVPMM
160 170 180 190 200
NIINGGSHAD NSVDIQEFMI MPVGFDCFER AVRACAEVYH ALKKTLNSKG
210 220 230 240 250
YSTGVGDEGG FAPNLKSNAE AIEVILEAIE KAGYEPGKEF FIAIDAASSE
260 270 280 290 300
YYKDGKYVLE HEGKTLTAAE MVDFFEDWVN KYPIISIEDG MAEEDWEGWK
310 320 330 340 350
LMTERLGKKV QLVGDDLFVT NTERLKTGIE KGIANSILIK LNQIGTLTET
360 370 380 390 400
LNAIEMANRA GYTAVVSHRS GETEDTTIAD LVVAVNAGQI KTGAPARSER
410 420 430
VAKYNQLIRI NEELGEVAEY RGRNAFFNLS K
Length:431
Mass (Da):46,963
Last modified:September 5, 2006 - v1
Checksum:i49A7FEAA1DD6A1B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000246 Genomic DNA Translation: ABG82939.1
RefSeqiWP_003460354.1, NC_008261.1

Genome annotation databases

EnsemblBacteriaiABG82939; ABG82939; CPF_1505
GeneIDi29571384
KEGGicpf:CPF_1505

Similar proteinsi

Entry informationi

Entry nameiENO_CLOP1
AccessioniPrimary (citable) accession number: Q0TQZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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