Reviewed, UniProtKB/Swiss-Prot Q0TQV3 (THIM_CLOP1)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	CPF_1544

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021505
Sites
	Binding site	36	1	Substrate; via amide nitrogen By similarity
	Binding site	112	1	ATP By similarity
	Binding site	160	1	ATP By similarity
	Binding site	187	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TQV3-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0E3E82BF9B81CD59	FASTA	265	29,495
	10 20 30 40 50 60 MEVLKRENPL IHMITNYVTV NDLAQVTINY GGLPLMATHH DELKEITKMA NGLLVNIGTL 70 80 90 100 110 120 EPYQMESSMI SMKIAKEKGI PSVLDPVGVQ ASKLRKDFAK KLILEGEPSL IKGNLAEIKT 130 140 150 160 170 180 LIGETSNSIG IDSFDDSLSE NTKNKIKEYA RERNLIVVVS GVVDFITNGE ESASVKNGTY 190 200 210 220 230 240 KMSKITGTGC MLGALLTLAL SFYDHKDLRF KEVVKAVSTW GICGELAEER LREKEGLMTF 250 260 KHNLLDELSI INDETIKERE KVIYD

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000246 Genomic DNA. Translation: ABG83081.1.
RefSeq	YP_695987.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4201531.
GenomeReviews	Gene locus CPF_1544 in contig CP000246_GR.
KEGG	cpf:CPF_1544.
TIGR	CPF_1544.
Phylogenomic databases
HOGENOM	Q0TQV3.
OMA	Q0TQV3. ASPVMAH.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_CLOP1

Accession

Primary (citable) accession number: Q0TQV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)