Reviewed, UniProtKB/Swiss-Prot Q0TQ60 (BIOD_CLOP1)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	CPF_1794

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 6-carboxyhexanoyl-CoA: step 3/4. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302499
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TQ60-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 03D45C5E3F5CB12F	FASTA	228	25,555
	10 20 30 40 50 60 MEKGVYIIGT STDIGKTFIS GLILKKLREE GRNAGYYKAV LSGAIKDKKG LIPLDCEEVM 70 80 90 100 110 120 EISGLKESYE NMVSYILENP YSPHLASEVE EVSISMEKIK KDYKSVRDKY DFILCEGSGG 130 140 150 160 170 180 IVCPISFSEK KLMLEDIIKE FNLPIILVSN SGLGAINHTV LTVSYLRNLG LKVKGIILNK 190 200 210 220 FNKSDIIHRD NKKIIKELTG VNNISTVPKI EDIEKYDLNE LNEVLYGI

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000246 Genomic DNA. Translation: ABG83122.1.
RefSeq	YP_696230.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4202332.
GenomeReviews	Gene locus CPF_1794 in contig CP000246_GR.
KEGG	cpf:CPF_1794.
TIGR	CPF_1794.
Phylogenomic databases
HOGENOM	Q0TQ60.
OMA	Q0TQ60. KGVFITA.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOP1

Accession

Primary (citable) accession number: Q0TQ60

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)