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Q0TQ59 (BIOB_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:CPF_1795
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Biotin synthase HAMAP-Rule MF_01694
PRO_0000381320

Sites

Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1061Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2681Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TQ59 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 833960A2DFD34BF7

FASTA31936,123
        10         20         30         40         50         60 
MDFVLKMKDK SLKNRKLTRE EGLRLFNSNL EELIKEANNI RKEIHGDGID LCSIINGKSG 

        70         80         90        100        110        120 
RCGEDCAFCA QSKYHKTNIS EYPLLDYEKI KKVAKENEDE GVHRFSIVTS GRGLYGEEFE 

       130        140        150        160        170        180 
RVITYYSNLN KELKINLCAS HGIINKESLI KLKKAGVKRY HHNLETSRNC YDKICKTHSY 

       190        200        210        220        230        240 
EERVKTIKNA KEAGLEVCSG GIIGLGETII DRIDLAITLR ELEIKSIPIN VLSAIKGTKL 

       250        260        270        280        290        300 
QHMIPLNEEE ILRTIAVFRF INPEAKIRLA GGRYLLKNFG ENAFKAGANA TITGNLLTTC 

       310 
GNKIKDDKRL IENIGMRIF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000246 Genomic DNA. Translation: ABG82486.1.
RefSeqYP_696231.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TQ59.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195103.CPF_1795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG82486; ABG82486; CPF_1795.
GeneID4201480.
KEGGcpf:CPF_1795.
PATRIC19485838. VBICloPer106549_1738.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycCPER195103:GHAW-1811-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CLOP1
AccessionPrimary (citable) accession number: Q0TQ59
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways