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Q0TQ16

- GLGB_CLOP1

UniProt

Q0TQ16 - GLGB_CLOP1

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene
glgB, CPF_1840
Organism
Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371Nucleophile By similarity
Active sitei390 – 3901Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciCPER195103:GHAW-1856-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:CPF_1840
OrganismiClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A)
Taxonomic identifieri195103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001823: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6596591,4-alpha-glucan branching enzyme GlgBUniRule annotationPRO_0000260645Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi195103.CPF_1840.

Structurei

3D structure databases

ProteinModelPortaliQ0TQ16.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TQ16-1 [UniParc]FASTAAdd to Basket

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MRNCKELKHE KNGNVTEKIG KNKGKSKKVS KDESLLSFDL FLEGKEHSAY    50
KFMGAHFVTE NRKRGVRFTT WAPRSSKIYV IGDFNNWELK EEYSMKKINE 100
RGIWSLFLPK LEEGIKYKFA VVNECGNNTV YKADPYAFKS ELRPNTASVL 150
TKIKSFRWGD KRWLNKREKE GLDNKPMNIY ELHLGSWKRK DGEFMTYEEI 200
SEVLVEYVKE MGYTHVEFMP INEHPLDASW GYQGVGYYSV TSRYGDLNGL 250
KALINKLHKN NIGVLLDWVP SHFCKDEHGL FMFDGSPTYE YGAWWKANNE 300
GWGTCNFDLG RPEVKSFLFS NAMYWINEFH IDGLRVDAVS NMLYLDYGRE 350
YGEWEPNIYG GNGNLEAIAF LKELNTIIKK EGKGAITVAE ESTSWEGITK 400
PVEEDGLGFD YKWNMGWMND TLSYIELDPI YRKYHHNKMN FSMMYNYSEK 450
FILPISHDEV VHGKKSLINK MWGDDWKKYA GLRLYASFMM GHPGKKLMFM 500
GCEFGQFVEW REWEELQWNI IEEFDIHKKT QEYFKALNHF YLENSSLWSL 550
DYEEEGFKWI DADNSEESVL SFIRIGKNKK EKLIFICNFT PEVYYDFKVG 600
VPELGEYVEV FNSDSLEFGG AGNIMGDSIL KATEESFKDF DYSIIVKVPP 650
LGTLVLKVK 659
Length:659
Mass (Da):77,008
Last modified:September 5, 2006 - v1
Checksum:i933BF043F0B4B065
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000246 Genomic DNA. Translation: ABG84928.1.
RefSeqiWP_011590903.1. NC_008261.1.
YP_696274.1. NC_008261.1.

Genome annotation databases

EnsemblBacteriaiABG84928; ABG84928; CPF_1840.
GeneIDi4201895.
KEGGicpf:CPF_1840.
PATRICi19485932. VBICloPer106549_1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000246 Genomic DNA. Translation: ABG84928.1 .
RefSeqi WP_011590903.1. NC_008261.1.
YP_696274.1. NC_008261.1.

3D structure databases

ProteinModelPortali Q0TQ16.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195103.CPF_1840.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG84928 ; ABG84928 ; CPF_1840 .
GeneIDi 4201895.
KEGGi cpf:CPF_1840.
PATRICi 19485932. VBICloPer106549_1785.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci CPER195103:GHAW-1856-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13124 / NCTC 8237 / Type A.

Entry informationi

Entry nameiGLGB_CLOP1
AccessioniPrimary (citable) accession number: Q0TQ16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 5, 2006
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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