Q0TNY3 (ASNA_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	CPF_2233

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017939

Sequences

	Sequence		Length	Mass (Da)
	Q0TNY3 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F1C03F3AE6EA0B44	FASTA	336	38,982
	10 20 30 40 50 60 MEKLFIPKGY KPLLSLRETE VAIKELKDFF EDSLAKNLNL TRVSAPLFVN KGSGLNDDLN 70 80 90 100 110 120 GIERPVSFDM KAMPEFNIQI VHSLAKWKRL ALHRYEFEHG EGLYTDMNAI RRDEDLDNIH 130 140 150 160 170 180 SIYVDQWDWE KIIDKEERNL ETLKETVRSI YGTFKATEDF IVAKYPHIEK ILPEDITFIT 190 200 210 220 230 240 SQELEDRYPD LTSKERETAI CKEFGAVFII GIGGKLASGE KHDDRSPDYD DWTLNGDLLF 250 260 270 280 290 300 YYPLFDEAVE LSSMGIRVDE ESLLKQLKIA ECEERKELPF HQMLLEGKLP YTIGGGIGQS 310 320 330 RICMFFLRKA HIGEVQASMW DEDMIRTCEE NNIHLL

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG83177.1.
RefSeq	YP_696657.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TNY3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TNY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4202640.
GenomeReviews	Gene locus CPF_2233 in contig CP000246_GR.
KEGG	cpf:CPF_2233.
PATRIC	19486718. VBICloPer106549_2173.
TIGR	CPF_2233.
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_2233-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_CLOP1

Accession

Primary (citable) accession number: Q0TNY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program