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Q0TNS2 (LEPA_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:CPF_2295
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000265649

Regions

Nucleotide binding17 – 226GTP By similarity
Nucleotide binding134 – 1374GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TNS2 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 800EF5750D229F70

FASTA60067,035
        10         20         30         40         50         60 
MDKKKYIRNF SIVAHIDHGK STLADRLLEK TGTLTQREME QQVLDTMELE KERGITIKSQ 

        70         80         90        100        110        120 
AARLIYKREN GEEYILNLID TPGHVDFTYE VSRSLAACEG AILVVDATQG IQAQTLANCY 

       130        140        150        160        170        180 
LALDNDLEIV PVINKVDLAS ARPDEIKQEI EDVIGIEAED APLVSAKTGL NIEDVLEEIV 

       190        200        210        220        230        240 
EKVPAPEGDE NAPLKALIFD SYYDSYKGVV CHIRVKDGKV KPGTKIKLMS TDKVYEVVET 

       250        260        270        280        290        300 
GVFTPALMPL KEGLSAGEVG YITASIKNVR DARVGDTVTE AARPTEEALP GYKPAIPMVY 

       310        320        330        340        350        360 
SGIYPVDGAK YEELKEALEK LQINDAALSF EPETSVALGF GFRCGFLGLL HMEIIQERVE 

       370        380        390        400        410        420 
REFNLDIITT APSVIYKVTK TNGESFDLTN PTNLPPMTEI AYMEEPVVKA SIITPTDYTG 

       430        440        450        460        470        480 
AVMELCQDRR GKFIDMQYLE ETRVVIHYEI PLNEIVYDFF DTLKSKTRGY ASLDYELKGY 

       490        500        510        520        530        540 
EQSKLVKLDI LLNGDNVDAL SMIVPEVKAY QRGRAIAEKL KEIIPRHMFE VPIQAAVGSK 

       550        560        570        580        590        600 
IIARETVKAM RKDVLAKCYG GDISRKKKLL EKQKEGKKRM RQLGTVEVPQ EAFMSVLKVD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000246 Genomic DNA. Translation: ABG84115.1.
RefSeqYP_696718.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TNS2.
SMRQ0TNS2. Positions 1-551.
ModBaseSearch...

Protein-protein interaction databases

STRING195103.CPF_2295.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG84115; ABG84115; CPF_2295.
GeneID4201955.
KEGGcpf:CPF_2295.
PATRIC19486842. VBICloPer106549_2234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0481.
HOGENOMHOG000020624.
KOK03596.
OMAKCYGGDA.
ProtClustDBPRK05433.

Enzyme and pathway databases

BioCycCPER195103:GHAW-2309-MONOMER.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]
PANTHERPTHR23115:SF40. PTHR23115:SF40. 1 hit.
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF54980. EFG_III_V. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF50447. Translat_factor. 1 hit.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_CLOP1
AccessionPrimary (citable) accession number: Q0TNS2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 5, 2006
Last modified: May 29, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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