Reviewed, UniProtKB/Swiss-Prot Q0TNR9 (GPR_CLOP1)

Names and origin

Protein names

Recommended name:
    Germination protease
    EC=3.4.24.78
Alternative name(s):
    Spore protease
    GPR endopeptidase
    Germination proteinase

Gene names

Name:	gpr
Ordered Locus Names:	CPF_2298

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Initiates the rapid degradation of small, acid-soluble proteins during spore germination By similarity.
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer By similarity.
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination By similarity.
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP spore germination Inferred from electronic annotation. Source: HAMAP
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 7	7	By similarity		PRO_0000316071
	Chain	8 – 325	318	Germination protease HAMAP MF_00626		PRO_1000051610

Sequences

	Sequence		Length	Mass (Da)
	Q0TNR9-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 9D8092C9C5A74F15	FASTA	325	35,548
	10 20 30 40 50 60 MYNVRTDLAV ESREIYKHRY NREIDGVVFE EKTVEEDIKV TNVDILNEEG AKAMGKPIGR 70 80 90 100 110 120 YVTIDIPEYT HYDGGIMDEV SHVVAASLEE LINLPEERTA LVVGLGNWNV TPDAIGPKVV 130 140 150 160 170 180 GKLMVTRHLK KVMPDIIDDS VRPVCAIAPG VLGITGIETG EIIKSLVEKI NPDLVVCIDA 190 200 210 220 230 240 LASRKLERVA RTIQISNTGI SPGAGVGNHR MQINEESLGI PVIALGVPTV VDAATIANDA 250 260 270 280 290 300 MDLVLDEMIN QADAGKEFYN ILNNIDKNEK GMMIKSLLDP YVGDLMVTPK EIDDIIESVS 310 320 KIIANGINIA LQPNMVLEDI NKFLN

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000246 Genomic DNA. Translation: ABG84499.1.
RefSeq	YP_696721.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4201287.
GenomeReviews	Gene locus CPF_2298 in contig CP000246_GR.
KEGG	cpf:CPF_2298.
TIGR	CPF_2298.
Phylogenomic databases
HOGENOM	Q0TNR9.
OMA	Q0TNR9. LMVTRHL.
Family and domain databases
HAMAP	MF_00626. [Tree]
InterPro	IPR005080. Peptidase_A25. [Graphical view]
Gene3D	G3DSA:3.40.50.10100. Peptidase_A25. 1 hit.
Pfam	PF03418. Peptidase_A25. 1 hit. [Graphical view]
PIRSF	PIRSF019549. Peptidase_A25. 1 hit.
ProDom	PD041835. Peptidase_A25. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01441. GPR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPR_CLOP1

Accession

Primary (citable) accession number: Q0TNR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)