Q0TNA4 (PYRG_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTP synthase
EC=6.3.4.2

Alternative name(s):
CTP synthetase
UTP--ammonia ligase

Gene names

Name:	pyrG
Ordered Locus Names:	CPF_2472

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP MF_01227
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP MF_01227
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity. HAMAP MF_01227
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	CTP synthase HAMAP MF_01227		PRO_0000266096
Regions
	Domain	295 – 535	241	Glutamine amidotransferase type-1
	Region	1 – 257	257	Aminator domain HAMAP MF_01227
Sites
	Active site	384	1	Nucleophile By similarity
	Active site	510	1	By similarity
	Active site	512	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TNA4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 2B6C15683AD14107	FASTA	535	59,656
	10 20 30 40 50 60 MSKNTKYVFV TGGVVSSLGK GITAASLGRL LKNRGLSVSI QKFDPYLNID PGTMSPYQHG 70 80 90 100 110 120 EVFVTDDGAE TDLDLGHYER FIDENLTQNS NVTSGRVYWS VISKERKGEY LGGTVQVIPH 130 140 150 160 170 180 ITNAIKDRVH RVGKERDVDV VITEIGGTIG DIESLPFLEA IRQIKYDVGK ENVCFIHVTL 190 200 210 220 230 240 VPYLGKAGEL KTKPTQHSVK ELRSIGIQPD IIVCRSEKEL SEDIKKKIGL FCNIDASEVI 250 260 270 280 290 300 QNLDAEHLYA VPLMLHKEGL DRLVCEKLGL GCRDIDNAEW IDMVHRITHL THTTKIALVG 310 320 330 340 350 360 KYVELHDAYI SVVEALNHGG LSNDTNVEIE WINAEDVTKE NVDELLSGVD GVLVPGGFGD 370 380 390 400 410 420 RGVEGKIEAI RWARENKKPF LGICLGMQCA VIEYARNVLG LEGAHSSELN PETPFPVIDL 430 440 450 460 470 480 MPEQKDVEDL GGTMRLGLYP CKLEDNTFCK DVYASDLIYE RHRHRYEFNN EYRTQLIESG 490 500 510 520 530 LTIAGTSPDG RLVECVEVKD HPWFVAVQYH PELKSRPNRP HPLFVGFVGA ALNNK

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13124 / NCTC 8237 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000246 Genomic DNA. Translation: ABG84508.1.
RefSeq	YP_696886.1. NC_008261.1.
3D structure databases
ProteinModelPortal	Q0TNA4.
SMR	Q0TNA4. Positions 4-535.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TNA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4203868.
GenomeReviews	Gene locus CPF_2472 in contig CP000246_GR.
KEGG	cpf:CPF_2472.
PATRIC	19487192. VBICloPer106549_2400.
TIGR	CPF_2472.
Phylogenomic databases
eggNOG	COG0504.
HOGENOM	HBG597806.
OMA	RVTMQKL.
ProtClustDB	PRK05380.
Enzyme and pathway databases
BioCyc	CPER195103:CPF_2472-MONOMER.
Family and domain databases
HAMAP	MF_01227. PyrG. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. [Graphical view]
KO	K01937.
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_CLOP1

Accession

Primary (citable) accession number: Q0TNA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program