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Q0TMM4 (SYC_CLOP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Ordered Locus Names:CPF_2737
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_0000332806

Regions

Motif30 – 4011"HIGH" region HAMAP-Rule MF_00041
Motif265 – 2695"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding281Zinc By similarity
Metal binding2081Zinc By similarity
Metal binding2331Zinc By similarity
Metal binding2371Zinc By similarity
Binding site2681ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0TMM4 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: B58F719D3F0E3220

FASTA46653,680
        10         20         30         40         50         60 
MIKVYNTLNK KKEEFIPLTP GEVKMYVCGP TVYNFFHIGN GRTFIVFDTI RRYFEYRGFK 

        70         80         90        100        110        120 
VDFVQNFTDI DDKMIKKANE EGTTVKKIGD TYIKEYYQDA DALNIERATV NPRATEFIGE 

       130        140        150        160        170        180 
IIKFVKGLVD KGYAYEVDGD VYFSTKKFEG YGKLSGQNIE DLQSGARISV DERKKDPMDF 

       190        200        210        220        230        240 
AIWKAQKPGE PAWNSPWGMG RPGWHIECSC MAKKLLGETI DIHAGGSDLK FPHHENEIAQ 

       250        260        270        280        290        300 
SEALTGEPFA RYWLHSAFVN VNNEKMSKSL NNFFTAREIL ERYDADVIRF LMLSAHYRQQ 

       310        320        330        340        350        360 
LNFSEDLLES AKASVERIYN AIGNLENLID EVSREEMNEE EKAYLESLNK YKEKYIEKMD 

       370        380        390        400        410        420 
DDFNTADAIT AIFDLIKDTN TNITIDSSKE LAQKALELIR ELGAPLGMFQ KSTKGNLEEE 

       430        440        450        460 
IEALIAKRQQ ARKDRDFTLA DKIRDELKDR GIVLEDTPQG VRWKMI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000246 Genomic DNA. Translation: ABG83340.1.
RefSeqYP_697116.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TMM4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195103.CPF_2737.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG83340; ABG83340; CPF_2737.
GeneID4203390.
KEGGcpf:CPF_2737.
PATRIC19487726. VBICloPer106549_2631.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0215.
HOGENOMHOG000245250.
KOK01883.
OMAMHADFDA.
OrthoDBEOG6RVFXC.

Enzyme and pathway databases

BioCycCPER195103:GHAW-2754-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_CLOP1
AccessionPrimary (citable) accession number: Q0TMM4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries