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Q0TMB1 (Q0TMB1_CLOP1) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003 EMBL ABG83791.1
Ordered Locus Names:CPF_2865
OrganismClostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195103 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1039 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
Q0TMB1 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 0FB571E377E1E3B1

FASTA1,039119,343
        10         20         30         40         50         60 
MYKKVELPKG FVGVEKEVAD LWKEKNIIKK NFDMNQDGEY FTFYDGPPTA NGKPHVGHVL 

        70         80         90        100        110        120 
TRVMKDIIPR YKVMKGYKVI RKAGWDTHGL PVELEIEKKL GISGKPQIED YGVEKFVTEC 

       130        140        150        160        170        180 
KDSVFTYVSM WEKMTEQIGY WVDMENPYVT YHNPYIESVW WALKQMWDKK LLYKGYKTMP 

       190        200        210        220        230        240 
YCPRCGTSLS SHEVSQGYKD VKDLTAVAKF KVKGEDNKYF LAWTTTPWTL PSNVALCINK 

       250        260        270        280        290        300 
AYDYVEAKQA EGDEIFILAK ELAPKVLGEG FEIVREFKGE ELLGMEYEQL LPFVTPTEGK 

       310        320        330        340        350        360 
AFVVVHGDYV TLSDGTGIVH IAPAYGEDDN LIGKQNGLAF LNLVDLSGNF VEEVTPWAGK 

       370        380        390        400        410        420 
FVKKCDQQIV DYLKEQNKLF KAEKHTHSYP HCWRCDTPLL YYPKESWFVA MTTLRDKLLA 

       430        440        450        460        470        480 
NNEKINWYPD NIKNGRFGKF LENVIDWGIS RDRYWGTPLP IWECECGHRE CIGSIEELKT 

       490        500        510        520        530        540 
KGINVPEDIE LHKPYIDKVH LNCPHCNKEM KRTAEVIDCW FDSGSMPFAQ HHYPFENKEL 

       550        560        570        580        590        600 
FEANYPAQFI SEAVDQTRGW FYTLLAISTA IFDRNPFENC IVLGHVLDKK GLKMSKSKGN 

       610        620        630        640        650        660 
VVDPFEVLDS QGADATRWHF YTASAPWLPT RFSIEDVAET QRKFLSTLWN VYSFYVLYAE 

       670        680        690        700        710        720 
LDSFNPLDYK DFVSDNVMDK WIMSKLNTLV KDVDDHLNNY RITQAALEIE EFTDELSNWY 

       730        740        750        760        770        780 
VRRNRARYWS EELTDDKIGA YTTLYRVLVT LCKVAAPFVP FITEEIYQNL VVNLDENALE 

       790        800        810        820        830        840 
SIHLGAWPEV DEKAIDKKLE EKMDLAYKIV KLGRSARNGA NIKNRQPLSK MLLSTSELPD 

       850        860        870        880        890        900 
YYGDIIKDEL NIKEVELGAD LSKYVNFEIK PNLPVLGRAY GKLIPAIRKE IASRNQMELA 

       910        920        930        940        950        960 
QKIQNGGVEV ITVDGNEIEL NAENLLVTMQ GLEGFAFAGE GSVGVVLDTT ITDELREEGH 

       970        980        990       1000       1010       1020 
VREIISKIQN MRKESGFEVA DKITLYVAEN DMLLDVIKKF EDVIKKETLT EEIVYNGDAD 

      1030 
YSEAKVNGEI LKMAVSKRA

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000246 Genomic DNA. Translation: ABG83791.1.
RefSeqYP_697229.1. NC_008261.1.

3D structure databases

ProteinModelPortalQ0TMB1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0TMB1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4203554.
GenomeReviewsGene locus CPF_2865 in contig CP000246_GR.
KEGGcpf:CPF_2865.
PATRIC19487988. VBICloPer106549_2745.
TIGRCPF_2865.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ0TMB1_CLOP1
AccessionPrimary (citable) accession number: Q0TMB1
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info