ID   GLPK_CLOP1              Reviewed;         500 AA.
AC   Q0TMA0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 26.
DE   RecName: Full=Glycerol kinase;
DE            EC=2.7.1.30;
DE   AltName: Full=ATP:glycerol 3-phosphotransferase;
DE   AltName: Full=Glycerokinase;
DE            Short=GK;
GN   Name=glpK; OrderedLocusNames=CPF_2876;
OS   Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195103;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R.,
RA   DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C.,
RA   Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J.,
RA   Sullivan S.A., Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S.,
RA   Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H.,
RA   Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I.,
RA   Melville S.B., Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial
RT   pathogen, Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
CC   -!- FUNCTION: Key enzyme in the regulation of glycerol uptake and
CC       metabolism.
CC   -!- CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3-
CC       phosphate.
CC   -!- PATHWAY: Polyol metabolism; glycerol degradation via glycerol
CC       kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
CC   -!- SIMILARITY: Belongs to the FGGY kinase family.
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DR   EMBL; CP000246; ABG82764.1; -; Genomic_DNA.
DR   RefSeq; YP_697240.1; -.
DR   SMR; Q0TMA0; 3-496.
DR   GeneID; 4200961; -.
DR   GenomeReviews; CP000246_GR; CPF_2876.
DR   KEGG; cpf:CPF_2876; -.
DR   TIGR; CPF_2876; -.
DR   HOGENOM; Q0TMA0; -.
DR   OMA; Q0TMA0; IAQREFT.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004370; F:glycerol kinase activity; IEA:HAMAP.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:HAMAP.
DR   HAMAP; MF_00186; -; 1.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018483; Carb_kinase_FGGY_CS.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   InterPro; IPR005999; Glycerol_kin.
DR   PANTHER; PTHR10196; FGGY_kin; 1.
DR   PANTHER; PTHR10196:SF9; Glycerol_kin; 1.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   TIGRFAMs; TIGR01311; glycerol_kin; 1.
DR   PROSITE; PS00933; FGGY_KINASES_1; 1.
DR   PROSITE; PS00445; FGGY_KINASES_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glycerol metabolism; Kinase;
KW   Nucleotide-binding; Transferase.
FT   CHAIN         1    500       Glycerol kinase.
FT                                /FTId=PRO_1000020722.
FT   NP_BIND     411    415       ATP (By similarity).
FT   BINDING      12     12       Substrate (By similarity).
FT   BINDING      16     16       ATP (By similarity).
FT   BINDING      82     82       Substrate (By similarity).
FT   BINDING     135    135       Substrate (By similarity).
FT   BINDING     245    245       Substrate (By similarity).
FT   BINDING     267    267       ATP (By similarity).
FT   BINDING     310    310       ATP; via carbonyl oxygen (By similarity).
SQ   SEQUENCE   500 AA;  56003 MW;  75EF0EF894CACF57 CRC64;
     MKKYIVALDQ GTTSSRAIIF DKEQNIIGVS QKEFNQIYPR EGWVEHDPME IWATQYSVLQ
     EVMAKCNITQ ENIAAIGITN QRETTIVWDK NTGVPIYNAI VWQCRRTADI CDELKERDGL
     VDYIRENTGL VLDAYFSGTK IKWILDNVEG AREKAEKGEL LFGTVDSWLV WKLTNGKVHV
     TDYTNASRTM IFNIKNLEWD ERMLKELDIP RSMLPEVKNS SEIYGYANLG AKGGIRVPIA
     GIAGDQQAAL FGQAAFNKGD VKNTYGTGCF LLMNTGEELV KSKSGLLTTI AIGLHGKVQY
     ALEGSVFVGG AVIQWLRDEL RIISDSSDTE YFATKVEDNG GVYVVPAFVG LGAPYWDMYA
     RGTIVGLTRG TNRNHIIRAA LESIAYQTRD VLEAMINDVG YDINCIKVDG GASRNNFLMQ
     FQSDLVGKKV IKPIITETTA LGAAYLAGLA VGYWSDKEEI AKLWFASEEF EPTISEERRN
     KYHKKWKKAV ERSKGWALED
//