Reviewed, UniProtKB/Swiss-Prot Q0TMA0 (GLPK_CLOP1)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	CPF_2876

Organism

Clostridium perfringens (strain ATCC 13124 / NCTC 8237 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195103 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000020722
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	310	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TMA0-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 75EF0EF894CACF57	FASTA	500	56,003
	10 20 30 40 50 60 MKKYIVALDQ GTTSSRAIIF DKEQNIIGVS QKEFNQIYPR EGWVEHDPME IWATQYSVLQ 70 80 90 100 110 120 EVMAKCNITQ ENIAAIGITN QRETTIVWDK NTGVPIYNAI VWQCRRTADI CDELKERDGL 130 140 150 160 170 180 VDYIRENTGL VLDAYFSGTK IKWILDNVEG AREKAEKGEL LFGTVDSWLV WKLTNGKVHV 190 200 210 220 230 240 TDYTNASRTM IFNIKNLEWD ERMLKELDIP RSMLPEVKNS SEIYGYANLG AKGGIRVPIA 250 260 270 280 290 300 GIAGDQQAAL FGQAAFNKGD VKNTYGTGCF LLMNTGEELV KSKSGLLTTI AIGLHGKVQY 310 320 330 340 350 360 ALEGSVFVGG AVIQWLRDEL RIISDSSDTE YFATKVEDNG GVYVVPAFVG LGAPYWDMYA 370 380 390 400 410 420 RGTIVGLTRG TNRNHIIRAA LESIAYQTRD VLEAMINDVG YDINCIKVDG GASRNNFLMQ 430 440 450 460 470 480 FQSDLVGKKV IKPIITETTA LGAAYLAGLA VGYWSDKEEI AKLWFASEEF EPTISEERRN 490 500 KYHKKWKKAV ERSKGWALED

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000246 Genomic DNA. Translation: ABG82764.1.
RefSeq	YP_697240.1.
3D structure databases
SMR	Q0TMA0. Positions 3-496.
ModBase	Search...
Genome annotation databases
GeneID	4200961.
GenomeReviews	Gene locus CPF_2876 in contig CP000246_GR.
KEGG	cpf:CPF_2876.
TIGR	CPF_2876.
Phylogenomic databases
HOGENOM	Q0TMA0.
OMA	Q0TMA0. IAQREFT.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_CLOP1

Accession

Primary (citable) accession number: Q0TMA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)