Reviewed, UniProtKB/Swiss-Prot Q0TKG6 (HEMH_ECOL5)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	ECP_0536

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ferrochelatase HAMAP MF_00323		PRO_1000019295
Sites
	Metal binding	194	1	Iron By similarity
	Metal binding	275	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TKG6-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 79E6F8BF465B1E7E	FASTA	320	35,942
	10 20 30 40 50 60 MRQTKTGILL ANLGTPDAPT PEAVKRYLKQ FLSDRRVVDT SRLLWWPLLR GVILPLRSPR 70 80 90 100 110 120 VAKLYASVWM EGGSPLMVYS RQQQQALAQR LPETPVALGM SYGSPSLESA VDELLAEHVD 130 140 150 160 170 180 HIVVLPLYPQ YSCSTVGAVW DELARILARK RSIPGISFIR DYADNHDYIN ALANSVRASF 190 200 210 220 230 240 AKHGEPDLLL LSYHGIPQRY ADEGDDYPQR CRTTTRELAS ALEMAPEKVM MTFQSRFGRE 250 260 270 280 290 300 PWLMPYTDET LKMLGEKGVG HIQVMCPGFA ADCLETLEEI AEQNREVFLG AGGKKYEYIP 310 320 ALNATPEHIE MMANLVAAYR

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG68565.1.
RefSeq	YP_668464.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4189243.
GenomeReviews	Gene locus ECP_0536 in contig CP000247_GR.
KEGG	ecp:ECP_0536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TKG6.
OMA	Q0TKG6. TIEEIGM.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_0536-MON.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_ECOL5

Accession

Primary (citable) accession number: Q0TKG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)