Reviewed, UniProtKB/Swiss-Prot Q0TJ41 (GHRA_ECOL5)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	ECP_1025

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348358
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TJ41-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 91CD0B8341C7A8C8	FASTA	312	35,354
	10 20 30 40 50 60 MDIIFYHPTF DTQWWIEALH KAIPQTRVRA WKSGDNESAD YALVWHPPVE MLAGRDLKAV 70 80 90 100 110 120 FALGAGVDSI LSKLQAHPEM LKPSVPLFRL EDTGMGEQMQ EYAVSQVLHW FRRFDDYRIQ 130 140 150 160 170 180 QNSSHWQPLP EYHREDFTIG ILGAGVLGSK VAQSLQTWRF PLRCWSRTRK SWPGVQSFAG 190 200 210 220 230 240 REELSAFLSQ CRVLINLLPN TPETVGIINQ QLLEKLPDGA YLLNLARGVH VVEDDLLAAL 250 260 270 280 290 300 DSGKVKGAML DVFNREPLPP ESPLWQHPRV TITPHVAAIT RPAEAVDYIS RTIAQVEKGE 310 RVCGQVDRAR GY

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG69038.1.
RefSeq	YP_668939.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4189414.
GenomeReviews	Gene locus ECP_1025 in contig CP000247_GR.
KEGG	ecp:ECP_1025.
NMPDR	fig|340197.3.peg.2530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TJ41.
OMA	Q0TJ41. YALVWRA.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_1025-MON.
Family and domain databases
HAMAP	MF_01666. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_ECOL5

Accession

Primary (citable) accession number: Q0TJ41

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)