Reviewed, UniProtKB/Swiss-Prot Q0THJ1 (PDXY_ECOL5)

Names and origin

Protein names

Recommended name:
    Pyridoxamine kinase
      Short name=PM kinase
    EC=2.7.1.35

Gene names

Name:	pdxY
Ordered Locus Names:	ECP_1581

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxamine By similarity.
Catalytic activity	ATP + pyridoxal = ADP + pyridoxal 5'-phosphate. HAMAP MF_01639
Sequence similarities	Belongs to the pyridoxine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP pyridoxal kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Pyridoxamine kinase HAMAP MF_01639		PRO_0000269810
Regions
	Nucleotide binding	182 – 183	2	ATP By similarity
	Nucleotide binding	208 – 223	16	ATP By similarity
Sites
	Binding site	10	1	Substrate By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0THJ1-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: D8061DBD92CE559A	FASTA	287	31,334
	10 20 30 40 50 60 MMKNILAIQS HVVYGHAGNS AAEFPMRRLG ANVWPLNTVQ FSNHTQYGKW TGCVMPPSHL 70 80 90 100 110 120 TEIVQGIAAI DKLHTCDAVL SGYLGSAEQG EHILGIVRQV KAANPQAKYF CDPVMGHPEK 130 140 150 160 170 180 GCIVAPGVAE FHVRHGLPAS DIIAPNLVEL EILCEHPVNN VEEAVLAARE LIAQGPQIVL 190 200 210 220 230 240 VKHLARAGYS RDRFEMLLVT ADEAWHISRP LVDFGMRQPV GVGDVTSGLL LVKLLQGATL 250 260 270 280 QEALEHVTAA VYEIMVTTKA MQEYELQVVA AQDRIANPEH YFSATKL

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG69588.1. Different initiation.
RefSeq	YP_669489.1.
3D structure databases
SMR	Q0THJ1. Positions 1-286.
ModBase	Search...
Genome annotation databases
GeneID	4190463.
GenomeReviews	Gene locus ECP_1581 in contig CP000247_GR.
KEGG	ecp:ECP_1581.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0THJ1.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_1581-MON.
Family and domain databases
HAMAP	MF_01639. [Tree]
InterPro	IPR013749. HMP-P_kinase-1. IPR004625. PyrdxlP_synth_PyrdxlKinase. [Graphical view]
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00687. pyridox_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXY_ECOL5

Accession

Primary (citable) accession number: Q0THJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 15, 2008
Last modified:	June 16, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)