ID   NADE_ECOL5              Reviewed;         275 AA.
AC   Q0TH90;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=ECP_1686;
OS   Escherichia coli O6:K15:H31 (strain 536 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=362663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16879640; DOI=10.1111/j.1365-2958.2006.05255.x;
RA   Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U.,
RA   Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.;
RT   "Role of pathogenicity island-associated integrases in the genome
RT   plasticity of uropathogenic Escherichia coli strain 536.";
RL   Mol. Microbiol. 61:584-595(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; CP000247; ABG69689.1; -; Genomic_DNA.
DR   RefSeq; YP_669590.1; -.
DR   SMR; Q0TH90; 2-275.
DR   GeneID; 4191320; -.
DR   GenomeReviews; CP000247_GR; ECP_1686.
DR   KEGG; ecp:ECP_1686; -.
DR   HOGENOM; Q0TH90; -.
DR   OMA; Q0TH90; ADLEEDR.
DR   BioCyc; ECOL362663:ECP_1686-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    275       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_1000077552.
FT   NP_BIND      46     53       ATP (By similarity).
FT   ACT_SITE     48     48       By similarity.
SQ   SEQUENCE   275 AA;  30653 MW;  268A4B34AF4F4583 CRC64;
     MTLQQQIIKA LGAKPQINAE EEIRRSIDFL KSYLQTYPFI KSLVLGISGG QDSTLAGKLC
     QMAINELRQE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ
     ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTSGVVVGT DHAAEAITGF FTKYGDGGTD
     INPLYRLNKR QGKQLLAALG CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG
     KNVPEQVART IENWYLKTEH KRRPPITVFD DFWKK
//