Q0TH90 (NADE_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	ECP_1686

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077552
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TH90 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 268A4B34AF4F4583	FASTA	275	30,653
	10 20 30 40 50 60 MTLQQQIIKA LGAKPQINAE EEIRRSIDFL KSYLQTYPFI KSLVLGISGG QDSTLAGKLC 70 80 90 100 110 120 QMAINELRQE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ 130 140 150 160 170 180 ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTSGVVVGT DHAAEAITGF FTKYGDGGTD 190 200 210 220 230 240 INPLYRLNKR QGKQLLAALG CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG 250 260 270 KNVPEQVART IENWYLKTEH KRRPPITVFD DFWKK

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 536 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000247 Genomic DNA. Translation: ABG69689.1.
RefSeq	YP_669590.1. NC_008253.1.
3D structure databases
ProteinModelPortal	Q0TH90.
SMR	Q0TH90. Positions 2-275.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TH90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000048878; EBESCP00000047067; EBESCG00000047927.
GeneID	4191320.
GenomeReviews	Gene locus ECP_1686 in contig CP000247_GR.
KEGG	ecp:ECP_1686.
PATRIC	18194106. VBIEscCol77757_1707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000009560.
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	Q0TH90.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_1686-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ECOL5

Accession

Primary (citable) accession number: Q0TH90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program