ID   ASTE_ECOL5              Reviewed;         322 AA.
AC   Q0TH86;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Succinylglutamate desuccinylase;
DE            EC=3.5.1.96;
GN   Name=astE; OrderedLocusNames=ECP_1690;
OS   Escherichia coli O6:K15:H31 (strain 536 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=362663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16879640; DOI=10.1111/j.1365-2958.2006.05255.x;
RA   Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U.,
RA   Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.;
RT   "Role of pathogenicity island-associated integrases in the genome
RT   plasticity of uropathogenic Escherichia coli strain 536.";
RL   Mol. Microbiol. 61:584-595(2006).
CC   -!- FUNCTION: Transforms N(2)-succinylglutamate into succinate and
CC       glutamate.
CC   -!- CATALYTIC ACTIVITY: N-succinyl-L-glutamate + H(2)O = succinate +
CC       L-glutamate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 5/5.
CC   -!- SIMILARITY: Belongs to the aspA/astE family. Succinylglutamate
CC       desuccinylase subfamily.
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DR   EMBL; CP000247; ABG69693.1; -; Genomic_DNA.
DR   RefSeq; YP_669594.1; -.
DR   SMR; Q0TH86; 1-322.
DR   GeneID; 4191324; -.
DR   GenomeReviews; CP000247_GR; ECP_1690.
DR   KEGG; ecp:ECP_1690; -.
DR   NMPDR; fig|340197.3.peg.1800; -.
DR   HOGENOM; Q0TH86; -.
DR   OMA; Q0TH86; EKFAIYP.
DR   BioCyc; ECOL362663:ECP_1690-MON; -.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:HAMAP.
DR   GO; GO:0009017; F:succinylglutamate desuccinylase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_00767; -; 1.
DR   InterPro; IPR007036; Aste_AspA.
DR   InterPro; IPR016681; SuccinylGlu_desuccinylase.
DR   Pfam; PF04952; AstE_AspA; 1.
DR   PIRSF; PIRSF017020; AstE; 1.
DR   TIGRFAMs; TIGR03242; arg_catab_astE; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase; Metal-binding;
KW   Zinc.
FT   CHAIN         1    322       Succinylglutamate desuccinylase.
FT                                /FTId=PRO_0000257712.
FT   ACT_SITE    210    210       Potential.
FT   METAL        53     53       Zinc (By similarity).
FT   METAL        56     56       Zinc (By similarity).
FT   METAL       147    147       Zinc (By similarity).
SQ   SEQUENCE   322 AA;  35813 MW;  BE02BAD60B3686EB CRC64;
     MDNFLALTLT GKKPVITERE INGVRWRWLG DGVLELTPLT PPQGALVISA GIHGNETAPV
     EMLDALLGAI SHGEIPLRWR LLVILGNPPA LKQGKRYCHS DMNRMFGGRW QLFAESGETC
     RARELEQCLE DFYDQGKESV RWHLDLHTAI RGSLHPQFGV LPQRDIPWDE KFLTWLGAAG
     LEALVFHQDP GGTFTHFSAR HFGALACTLE LGKALPFGQN DLRQFAVTAS AIAALLSGES
     VGIVRTPPLR YRVVSQITRH SPSFEMHMAN DTLNFMPFEK GTLLAQDGEE RFTVTHDVEY
     VLFPNPLVAL GLRAGLMLEK IS
//