UniProtKB - Q0TGZ7 (RSMF_ECOL5)
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Protein
Ribosomal RNA small subunit methyltransferase F
Gene
rsmF
Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Functioni
Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation
Catalytic activityi
S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 149 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 176 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 194 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Active sitei | 247 | NucleophileUniRule annotation | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- rRNA methyltransferase activity Source: InterPro
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | ECOL362663:G1G5S-1898-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)Alternative name(s): 16S rRNA m5C1407 methyltransferaseUniRule annotation rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation |
Gene namesi | Name:rsmFUniRule annotation Ordered Locus Names:ECP_1779 |
Organismi | Escherichia coli O6:K15:H31 (strain 536 / UPEC) |
Taxonomic identifieri | 362663 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285001 | 1 – 479 | Ribosomal RNA small subunit methyltransferase FAdd BLAST | 479 |
Structurei
3D structure databases
ProteinModelPortali | Q0TGZ7 |
SMRi | Q0TGZ7 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 131 | S-adenosyl-L-methionine bindingUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105CYJ Bacteria COG0144 LUCA COG3270 LUCA |
HOGENOMi | HOG000218115 |
KOi | K11392 |
OMAi | DPLFHAG |
Family and domain databases
HAMAPi | MF_01579 16SrRNA_methyltr_F, 1 hit |
InterProi | View protein in InterPro IPR018314 Fmu/NOL1/Nop2p_CS IPR031341 Methyltr_RsmF_N IPR001678 MeTrfase_RsmB/NOP2 IPR027391 Nol1_Nop2_Fmu_2 IPR011023 Nop2p IPR023267 RCMT IPR023545 rRNA_ssu_MeTfrase_F IPR029063 SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189 Methyltr_RsmB-F, 1 hit PF17125 Methyltr_RsmF_N, 1 hit PF13636 Methyltranf_PUA, 1 hit |
PRINTSi | PR02008 RCMTFAMILY |
SUPFAMi | SSF53335 SSF53335, 1 hit |
TIGRFAMsi | TIGR00446 nop2p, 1 hit |
PROSITEi | View protein in PROSITE PS01153 NOL1_NOP2_SUN, 1 hit PS51686 SAM_MT_RSMB_NOP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q0TGZ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV
60 70 80 90 100
ADFLQLTAPY GWTLTPIPWC EEGFWIERYN EDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFANGNAPQR VMDVAAAPGS KTTQIAARMN NEGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
REENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TQAIPALPTP KYKVGNFPFS PVKDREAGQI RQAAASVGLN
360 370 380 390 400
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE
410 420 430 440 450
AVIALASPDN VNAFELTPQE AEEWYRGRDV YPHAAPVADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGNA
Sequence cautioni
The sequence ABG69782 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000247 Genomic DNA Translation: ABG69782.1 Different initiation. |
RefSeqi | WP_024174322.1, NC_008253.1 |
Genome annotation databases
EnsemblBacteriai | ABG69782; ABG69782; ECP_1779 |
KEGGi | ecp:ECP_1779 |
Similar proteinsi
Entry informationi
Entry namei | RSMF_ECOL5 | |
Accessioni | Q0TGZ7Primary (citable) accession number: Q0TGZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | May 1, 2007 | |
Last modified: | February 28, 2018 | |
This is version 76 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |