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Q0TGU0

- OTSA_ECOL5

UniProt

Q0TGU0 - OTSA_ECOL5

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei77 – 771Glucose-6-phosphateBy similarity
    Sitei86 – 861Involved in alpha anomer selectivityBy similarity
    Binding sitei131 – 1311Glucose-6-phosphateBy similarity
    Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
    Binding sitei263 – 2631UDP-glucoseBy similarity
    Binding sitei268 – 2681UDP-glucoseBy similarity
    Binding sitei301 – 3011Glucose-6-phosphateBy similarity
    Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciECOL362663:GIY5-1844-MONOMER.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:ECP_1838
    OrganismiEscherichia coli O6:K15:H31 (strain 536 / UPEC)
    Taxonomic identifieri362663 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000009182: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348900Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi362663.ECP_1838.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0TGU0.
    SMRiQ0TGU0. Positions 2-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 232UDP-glucose bindingBy similarity
    Regioni366 – 3705UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191478.
    KOiK00697.
    OMAiWGESFVK.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0TGU0-1 [UniParc]FASTAAdd to Basket

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    MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED    50
    QPLKKVKKGN ITWASFNLSE QDLDEYYNKF SNAVLWPAFH YRLDLVQFQR 100
    PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI 150
    GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN 200
    LTRVTTRSAK SHTACGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA 250
    ELKNVQNIFS VERLDYSKGL PERFLAYEAL LEKYPQHHGK IRYTQIAPTS 300
    RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR 350
    YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI 400
    VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD 450
    LKQIVPRSAE SQQRDKVATF PKLA 474
    Length:474
    Mass (Da):53,528
    Last modified:September 5, 2006 - v1
    Checksum:iC81B480CF458A7FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000247 Genomic DNA. Translation: ABG69839.1.
    RefSeqiYP_669740.1. NC_008253.1.

    Genome annotation databases

    EnsemblBacteriaiABG69839; ABG69839; ECP_1838.
    GeneIDi4189540.
    KEGGiecp:ECP_1838.
    PATRICi18194438. VBIEscCol77757_1873.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000247 Genomic DNA. Translation: ABG69839.1 .
    RefSeqi YP_669740.1. NC_008253.1.

    3D structure databases

    ProteinModelPortali Q0TGU0.
    SMRi Q0TGU0. Positions 2-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 362663.ECP_1838.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG69839 ; ABG69839 ; ECP_1838 .
    GeneIDi 4189540.
    KEGGi ecp:ECP_1838.
    PATRICi 18194438. VBIEscCol77757_1873.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191478.
    KOi K00697.
    OMAi WGESFVK.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .
    BioCyci ECOL362663:GIY5-1844-MONOMER.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
      Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
      Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 536 / UPEC.

    Entry informationi

    Entry nameiOTSA_ECOL5
    AccessioniPrimary (citable) accession number: Q0TGU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3