Reviewed, UniProtKB/Swiss-Prot Q0TFZ6 (GATY_ECOL5)

Names and origin

Protein names

Recommended name:
    D-tagatose-1,6-bisphosphate aldolase subunit gatY
      Short name=TagBP aldolase
      Short name=TBPA
    EC=4.1.2.40
Alternative name(s):
    Tagatose-bisphosphate aldolase
    D-tagatose-bisphosphate aldolase class II

Gene names

Name:	gatY
Ordered Locus Names:	ECP_2134

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the tagatose-1,6-bisphosphate aldolase gatYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-bisphosphate (TBP). Requires gatZ subunit for full activity and stability. Is involved in the catabolism of galactitol By similarity.
Catalytic activity	D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP MF_01294
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 2/2. HAMAP MF_01294
Subunit structure	Forms a complex with gatZ By similarity.
Sequence similarities	Belongs to the class II fructose-bisphosphate aldolase family. TagBP aldolase gatY subfamily.

Ontologies

Keywords
Biological process	Galactitol metabolism
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	galactitol catabolic process Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: InterPro lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: InterPro tagatose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	D-tagatose-1,6-bisphosphate aldolase subunit gatY HAMAP MF_01294		PRO_0000355337
Regions
	Region	209 – 211	3	Dihydroxyacetone phosphate binding By similarity
	Region	230 – 233	4	Dihydroxyacetone phosphate binding By similarity
Sites
	Active site	82	1	Proton donor By similarity
	Metal binding	83	1	Zinc; catalytic By similarity
	Metal binding	180	1	Zinc; catalytic By similarity
	Metal binding	208	1	Zinc; catalytic By similarity
	Binding site	181	1	Dihydroxyacetone phosphate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TFZ6-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D41E361BCCC3B1B2	FASTA	284	30,980
	10 20 30 40 50 60 MYVVSTKQML NNAQRGGYAV PAFNIHNLET MQVVVETAAS MHAPVIIAGT PGTFTHVGTE 70 80 90 100 110 120 NLMALVSAMA KQYHHPLAIH LDHHTKFDDI AQKVRSGVRS VMIDASHLPF AQNISRVKEV 130 140 150 160 170 180 VDFCHRFDVS VEAELGQLGG QEDDVQVNEA DAFYTNPVQA REFAEATGID SLAVAIGTAH 190 200 210 220 230 240 GMYARAPALD FSRLENIRQW VNLPLVLHGA SGLSTKDIQQ TIKLGICKIN VATELKNAFS 250 260 270 280 QALKNYLTEH PEATDPRDYL QSAKSAMRDV VSKVIADCGC EGRA

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG70133.1.
RefSeq	YP_670034.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4191685.
GenomeReviews	Gene locus ECP_2134 in contig CP000247_GR.
KEGG	ecp:ECP_2134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TFZ6.
OMA	Q0TFZ6. VNEADAF.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_2134-MON.
Family and domain databases
HAMAP	MF_01294. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000771. Ketose_bisP_aldolase_II. IPR011288. Tag_bisphos_ald. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01116. F_bP_aldolase. 1 hit. [Graphical view]
PIRSF	PIRSF001359. F_bP_aldolase_II. 1 hit.
ProDom	PD002376. K_bP_aldolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00167. cbbA. 1 hit. TIGR01858. tag_bisphos_ald. 1 hit.
PROSITE	PS00602. ALDOLASE_CLASS_II_1. 1 hit. PS00806. ALDOLASE_CLASS_II_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATY_ECOL5

Accession

Primary (citable) accession number: Q0TFZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)