ID   THIM_ECOL5              Reviewed;         262 AA.
AC   Q0TFY8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Hydroxyethylthiazole kinase;
DE            EC=2.7.1.50;
DE   AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase;
DE            Short=Thz kinase;
DE            Short=TH kinase;
GN   Name=thiM; OrderedLocusNames=ECP_2142;
OS   Escherichia coli O6:K15:H31 (strain 536 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=362663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16879640; DOI=10.1111/j.1365-2958.2006.05255.x;
RA   Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U.,
RA   Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.;
RT   "Role of pathogenicity island-associated integrases in the genome
RT   plasticity of uropathogenic Escherichia coli strain 536.";
RL   Mol. Microbiol. 61:584-595(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole =
CC       ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate
CC       biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-
CC       5-(2-hydroxyethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the Thz kinase family.
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DR   EMBL; CP000247; ABG70141.1; -; Genomic_DNA.
DR   RefSeq; YP_670042.1; -.
DR   GeneID; 4187647; -.
DR   GenomeReviews; CP000247_GR; ECP_2142.
DR   KEGG; ecp:ECP_2142; -.
DR   NMPDR; fig|340197.3.peg.162; -.
DR   HOGENOM; Q0TFY8; -.
DR   OMA; Q0TFY8; ASPVMAH.
DR   BioCyc; ECOL362663:ECP_2142-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:HAMAP.
DR   GO; GO:0009228; P:thiamin biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00228; -; 1.
DR   InterPro; IPR000417; Hyethyz_kinase.
DR   InterPro; IPR011144; Hyethyz_kinsmonf.
DR   PANTHER; PTHR20857:SF14; Hyethyz_kinase; 1.
DR   Pfam; PF02110; HK; 1.
DR   PIRSF; PIRSF000513; Thz_kinase; 1.
DR   PRINTS; PR01099; HYETHTZKNASE.
DR   TIGRFAMs; TIGR00694; thiM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Kinase; Nucleotide-binding;
KW   Thiamine biosynthesis; Transferase.
FT   CHAIN         1    262       Hydroxyethylthiazole kinase.
FT                                /FTId=PRO_1000021510.
FT   BINDING      50     50       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     125    125       ATP (By similarity).
FT   BINDING     171    171       ATP (By similarity).
FT   BINDING     198    198       Substrate; via amide nitrogen (By
FT                                similarity).
SQ   SEQUENCE   262 AA;  27313 MW;  C3F974570B7B60FF CRC64;
     MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASHF
     AAIASALLIN VGTLTQPRAQ AMSAAVEQAT RSQTPWTLDP VAVGALDYRR RFCVELLSHK
     PTAIRGNASE IMALAGVANG GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEVDYVTDG
     RRTVGIHGGD PLMTKVVGTG CALSAVVAAC CALPGDTLEN IASACHWMKQ AGERAVARSE
     GPGSFVPHFL DALWQLTQEV QA
//