Q0TFL0 (UBIG_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-demethylubiquinone-9 3-methyltransferase
EC=2.1.1.64

Alternative name(s):
2-polyprenyl-6-hydroxyphenyl methylase
EC=2.1.1.222
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
Short name=DHHB methyltransferase

Gene names

Name:	ubiG
Ordered Locus Names:	ECP_2275

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-specific O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway By similarity.
Catalytic activity	S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. HAMAP MF_00472 S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol.
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_00472
Sequence similarities	Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: InterPro 3-demethylubiquinone-9 3-O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	3-demethylubiquinone-9 3-methyltransferase HAMAP MF_00472		PRO_1000013899

Sequences

	Sequence		Length	Mass (Da)
	Q0TFL0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 93F5364A1E1F911F	FASTA	240	26,573
	10 20 30 40 50 60 MNAEKSPVNH NVDHKEIAKF EAVASRWWDL EGEFKPLHRI NPLRLGYIAE RAGGLFGKKV 70 80 90 100 110 120 LDVGCGGGIL AESMAREGAT VTGLDMGFEP LQVAKLHALE SGIQVDYVQE TVEEHAAKHA 130 140 150 160 170 180 GQYDVVTCME MLEHVPDPQS VVRACAQLVK PGGDVFFSTL NRNGKSWLMA VVGAEYILRM 190 200 210 220 230 240 VPKGTHDVKK FIKPAELLGW VDQTSLKERH MTGLHYNPIT NTFKLGPGVD VNYMLHTQNK

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 536 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000247 Genomic DNA. Translation: ABG70269.1.
RefSeq	YP_670170.1. NC_008253.1.
3D structure databases
ProteinModelPortal	Q0TFL0.
SMR	Q0TFL0. Positions 46-156.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TFL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000048777; EBESCP00000046966; EBESCG00000047826.
GeneID	4191924.
GenomeReviews	Gene locus ECP_2275 in contig CP000247_GR.
KEGG	ecp:ECP_2275.
NMPDR	fig|340197.3.peg.297.
PATRIC	18195311. VBIEscCol77757_2299.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2227.
GeneTree	EBGT00050000009368.
HOGENOM	HBG738842.
OMA	DHAEIAK.
PhylomeDB	Q0TFL0.
ProtClustDB	PRK05134.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_2275-MONOMER.
Family and domain databases
HAMAP	MF_00472. UbiG. [Tree]
InterPro	IPR013216. Methyltransf_11. IPR010233. UbiG_MeTrfase. [Graphical view]
KO	K00568.
PANTHER	PTHR23134. PTHR23134. 1 hit.
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR01983. UbiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIG_ECOL5

Accession

Primary (citable) accession number: Q0TFL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program