Reviewed, UniProtKB/Swiss-Prot Q0TFK1 (GLPB_ECOL5)

Names and origin

Protein names

Recommended name:
    Anaerobic glycerol-3-phosphate dehydrogenase subunit B
      Short name=Anaerobic G-3-P dehydrogenase subunit B
      Short name=Anaerobic G3Pdhase B
    EC=1.1.5.3

Gene names

Name:	glpB
Ordered Locus Names:	ECP_2284

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor By similarity.
Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol. HAMAP MF_00753
Cofactor	FMN By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. HAMAP MF_00753
Subunit structure	Composed of a catalytic glpA/B dimer and of membrane bound glpC By similarity.
Sequence similarities	Belongs to the anaerobic G-3-P dehydrogenase subunit B family.
Sequence caution	The sequence ABG70278.1 differs from that shown. Reason: Erroneous termination at position 394. Translated as Gly.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Anaerobic glycerol-3-phosphate dehydrogenase subunit B HAMAP MF_00753		PRO_0000258901

Sequences

	Sequence		Length	Mass (Da)
	Q0TFK1-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 8CC9C8ADE0ADB2E7	FASTA	419	45,393
	10 20 30 40 50 60 MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH LPDGQPVTEI 70 80 90 100 110 120 HSGLESLRQQ APAHPYTLLG PQRVLDLACQ AQALIAESGA QLQGSVELAH QRITPLGTLR 130 140 150 160 170 180 STWLSSPEVP VWPLPAKKIC VVGISGLMDF QAHLAAASLR ELDLKVETAE IELPELDVLR 190 200 210 220 230 240 NNATEFRAVN IARFLDNEEN WPLLLDALIP VANTCEMILM PACFGLANDK LWHWLNEKLP 250 260 270 280 290 300 CSLMLLPTLP PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK KVTCKNGVVN EIWTRNHADI 310 320 330 340 350 360 PLRPRFAVLA SGSFFSGGLV AERDGIREPI LGLDVLQTAT RGEWYKGDFF APQPWQQFGV 370 380 390 400 410 TTDEALRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC AVSALHAAQQ IAQRAGGQQ

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG70278.1. Sequence problems.
RefSeq	YP_670179.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4191933.
GenomeReviews	Gene locus ECP_2284 in contig CP000247_GR.
KEGG	ecp:ECP_2284.
NMPDR	fig|340197.3.peg.306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TFK1.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_2284-MON.
Family and domain databases
HAMAP	MF_00753. [Tree]
InterPro	IPR009158. Anaerobic_glycerol3P_DH_bsu. IPR003953. FAD_bind2_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
PIRSF	PIRSF000141. Anaerobic_G3P_dh. 1 hit.
TIGRFAMs	TIGR03378. glycerol3P_GlpB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLPB_ECOL5

Accession

Primary (citable) accession number: Q0TFK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	March 24, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)