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Q0TFH7

- MEND_ECOL5

UniProt

Q0TFH7 - MEND_ECOL5

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Protein
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Gene
menD, ECP_2308
Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity.UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese By similarity.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciECOL362663:GIY5-2320-MONOMER.
UniPathwayiUPA00079; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (EC:2.2.1.9)
Short name:
SEPHCHC synthase
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene namesi
Name:menD
Ordered Locus Names:ECP_2308
OrganismiEscherichia coli O6:K15:H31 (strain 536 / UPEC)
Taxonomic identifieri362663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000009182: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5565562-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation
PRO_0000341745Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi362663.ECP_2308.

Structurei

3D structure databases

ProteinModelPortaliQ0TFH7.
SMRiQ0TFH7. Positions 1-556.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218360.
KOiK02551.
OMAiQKPWLLE.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TFH7-1 [UniParc]FASTAAdd to Basket

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MSVSAFNRRW AAVILEALTR HGVRHICIAP GSRSTPLTLA AAENSAFIHH    50
THFDERGLGH LALGLAKVSK QPVAVIVTSG TAVANLYPAL IEAGLTGEKL 100
ILLTADRPPE LIDCGSNQAI RQPGMFASHP THSISLPRPT QDIPARWLVS 150
TIDHALGTLH AGGVHINCPF AEPLYGEMDD TGISWQQRLG DWWQDDKPWL 200
REAPRRESEK QRDWFFWRQK RGVVVAGRMS AEEGKKVALW AQTLGWPLIG 250
DVLSQTGQPL PCADLWLGNA KATSELQQAQ IVVQLGSSLT GKRLLQWQAS 300
CEPEEYWIVD DIEGRLDPAH HRGRRLIANI ADWLELHPAE KRQPWCVEIP 350
RLAEQAMQAV IARRDAFGEA QLAHRISDYL PEQGQLFVGN SLVVRLIDAL 400
SQLPAGYPVY SNRGASGIDG LLSTAAGVQR ASGKPTLAIV GDLSALYDLN 450
ALALLRQVSA PLVLIVVNNN GGQIFSLLPT PKSERERFYL MPQNVHFEHA 500
AAMFELKYHR PQNWQELETT LVDAWRTPTT TVIEMVVNDT DGAQTLQQLL 550
AQVSHL 556
Length:556
Mass (Da):61,434
Last modified:July 1, 2008 - v2
Checksum:i1AC61DE58A0B4DC4
GO

Sequence cautioni

The sequence ABG70302.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG70302.1. Different initiation.
RefSeqiYP_670203.1. NC_008253.1.

Genome annotation databases

EnsemblBacteriaiABG70302; ABG70302; ECP_2308.
GeneIDi4191444.
KEGGiecp:ECP_2308.
PATRICi18195383. VBIEscCol77757_2335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG70302.1 . Different initiation.
RefSeqi YP_670203.1. NC_008253.1.

3D structure databases

ProteinModelPortali Q0TFH7.
SMRi Q0TFH7. Positions 1-556.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362663.ECP_2308.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG70302 ; ABG70302 ; ECP_2308 .
GeneIDi 4191444.
KEGGi ecp:ECP_2308.
PATRICi 18195383. VBIEscCol77757_2335.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218360.
KOi K02551.
OMAi QKPWLLE.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 ; UER00164 .
BioCyci ECOL362663:GIY5-2320-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
    Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
    Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 536 / UPEC.

Entry informationi

Entry nameiMEND_ECOL5
AccessioniPrimary (citable) accession number: Q0TFH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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