Q0TEA8 (CYSC_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylyl-sulfate kinase
EC=2.7.1.25

Alternative name(s):
APS kinase
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase

Gene names

Name:	cysC
Ordered Locus Names:	ECP_2732

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate. HAMAP MF_00065
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate. HAMAP MF_00065
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. HAMAP MF_00065
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylylsulfate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Adenylyl-sulfate kinase HAMAP MF_00065		PRO_1000009011
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity
Sites
	Active site	109	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TEA8 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 5ABD192EE36679BB	FASTA	201	22,320
	10 20 30 40 50 60 MALHDENVVW HSHPVTPQQR EQHHGHRGVV LWFTGLSGSG KSTVAGGLEE ALHKLGVSTY 70 80 90 100 110 120 LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG LVVLTAFISP HRAERQMVRE 130 140 150 160 170 180 RVGEGRFIEV FVDTPLAICE ARDPKGLYKK ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ 190 200 LVTNLVQQLL DLLRQNDIIR S

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 536 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000247 Genomic DNA. Translation: ABG70721.1.
RefSeq	YP_670622.1. NC_008253.1.
3D structure databases
ProteinModelPortal	Q0TEA8.
SMR	Q0TEA8. Positions 14-200.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TEA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000050223; EBESCP00000048412; EBESCG00000049271.
GeneID	4191557.
GenomeReviews	Gene locus ECP_2732 in contig CP000247_GR.
KEGG	ecp:ECP_2732.
PATRIC	18196288. VBIEscCol77757_2771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0529.
GeneTree	EBGT00050000011347.
HOGENOM	HBG309946.
OMA	AELGYHT.
PhylomeDB	Q0TEA8.
ProtClustDB	PRK03846.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_2732-MONOMER.
Family and domain databases
HAMAP	MF_00065. Adenylyl_sulf_kinase. [Tree]
InterPro	IPR002891. APS_kinase_C. [Graphical view]
KO	K00860.
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00455. ApsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSC_ECOL5

Accession

Primary (citable) accession number: Q0TEA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program