Reviewed, UniProtKB/Swiss-Prot Q0TEA8 (CYSC_ECOL5)

Names and origin

Protein names

Recommended name:
    Adenylyl-sulfate kinase
    EC=2.7.1.25
Alternative name(s):
    APS kinase
    Adenosine-5'-phosphosulfate kinase
    ATP adenosine-5'-phosphosulfate 3'-phosphotransferase

Gene names

Name:	cysC
Ordered Locus Names:	ECP_2732

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate. HAMAP MF_00065
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate. HAMAP MF_00065
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. HAMAP MF_00065
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP adenylylsulfate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Adenylyl-sulfate kinase HAMAP MF_00065		PRO_1000009011
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity
Sites
	Active site	109	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TEA8-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 5ABD192EE36679BB	FASTA	201	22,320
	10 20 30 40 50 60 MALHDENVVW HSHPVTPQQR EQHHGHRGVV LWFTGLSGSG KSTVAGGLEE ALHKLGVSTY 70 80 90 100 110 120 LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG LVVLTAFISP HRAERQMVRE 130 140 150 160 170 180 RVGEGRFIEV FVDTPLAICE ARDPKGLYKK ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ 190 200 LVTNLVQQLL DLLRQNDIIR S

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG70721.1.
RefSeq	YP_670622.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4191557.
GenomeReviews	Gene locus ECP_2732 in contig CP000247_GR.
KEGG	ecp:ECP_2732.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TEA8.
OMA	Q0TEA8. IITITAF.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_2732-MON.
Family and domain databases
HAMAP	MF_00065. [Tree]
InterPro	IPR002891. APS_kinase_C. [Graphical view]
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
ProDom	PD002350. APS_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00455. apsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSC_ECOL5

Accession

Primary (citable) accession number: Q0TEA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)