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Q0TD22 (RLMG_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase G

EC=2.1.1.174
Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG
Gene names
Name:rlmG
Ordered Locus Names:ECP_3175
OrganismEscherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier362663 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP-Rule MF_01859

Catalytic activity

S-adenosyl-L-methionine + guanine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1835) in 23S rRNA. HAMAP-Rule MF_01859

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01859.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(1835)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Ribosomal RNA large subunit methyltransferase G HAMAP-Rule MF_01859
PRO_0000366464

Sequences

Sequence LengthMass (Da)Tools
Q0TD22 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 15BB5B3E8B8BE9CE

FASTA37842,339
        10         20         30         40         50         60 
MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI LNDAFGALSC 

        70         80         90        100        110        120 
ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST ADYPQQPGVV LIKVPKTLAL 

       130        140        150        160        170        180 
LEQQLRALRK VVTPDTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFNE 

       190        200        210        220        230        240 
PPLVDAPQTV SWKLEGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG 

       250        260        270        280        290        300 
VIGLTLLDKN PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN 

       310        320        330        340        350        360 
AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI 

       370 
ATNNKFVVLK AVKLGRRR 

« Hide

References

[1]"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 536 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000247 Genomic DNA. Translation: ABG71157.1.
RefSeqYP_671058.1. NC_008253.1.

3D structure databases

ProteinModelPortalQ0TD22.
SMRQ0TD22. Positions 27-372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362663.ECP_3175.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG71157; ABG71157; ECP_3175.
GeneID4188456.
KEGGecp:ECP_3175.
PATRIC18197192. VBIEscCol77757_3215.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2813.
HOGENOMHOG000246323.
KOK11391.
OMANRHLGYH.
OrthoDBEOG67X1ZC.
PhylomeDBQ0TD22.
ProtClustDBPRK15001.

Enzyme and pathway databases

BioCycECOL362663:GIY5-3197-MONOMER.

Family and domain databases

HAMAPMF_01859. 23SrRNA_methyltr_G.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR017237. rRNA_m2G-MeTrfase_RsmD.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamPF05175. MTS. 1 hit.
[Graphical view]
PIRSFPIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLMG_ECOL5
AccessionPrimary (citable) accession number: Q0TD22
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 5, 2006
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families