Reviewed, UniProtKB/Swiss-Prot Q0TCY1 (GARL_ECOL5)

Names and origin

Protein names

Recommended name:
    5-keto-4-deoxy-D-glucarate aldolase
      Short name=KDGluc aldolase
      Short name=KDGlucA
    EC=4.1.2.20
Alternative name(s):
    5-dehydro-4-deoxy-D-glucarate aldolase
    2-keto-3-deoxy-D-glucarate aldolase
    2-dehydro-3-deoxy-D-glucarate aldolase
    Alpha-keto-beta-deoxy-D-glucarate aldolase

Gene names

Name:	garL
Ordered Locus Names:	ECP_3216

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde By similarity.
Catalytic activity	5-dehydro-4-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate acid metabolism; D-galactaric acid degradation; D-glyceric acid from D-galactaric acid: step 2/3. HAMAP MF_01291
Subunit structure	Homohexamer; trimer of dimers By similarity.
Sequence similarities	Belongs to the hpcH/hpaI aldolase family. KDGluc aldolase subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-galactarate catabolic process Inferred from electronic annotation. Source: HAMAP cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro glucarate catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-dehydro-3-deoxyglucarate aldolase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	5-keto-4-deoxy-D-glucarate aldolase HAMAP MF_01291		PRO_0000353150
Sites
	Active site	50	1	Proton acceptor By similarity
	Metal binding	153	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	178	1	Substrate; via amide nitrogen By similarity
	Binding site	179	1	Substrate By similarity
	Site	75	1	Transition state stabilizer By similarity
	Site	89	1	Increases basicity of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TCY1-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A363593FFB171401	FASTA	256	27,399
	10 20 30 40 50 60 MNNDVFPNKF KAALAAKQVQ IGCWSALSNP ISTEVLGLAG FDWLVLDGEH APNDISTFIP 70 80 90 100 110 120 QLMALKGSAS APVVRVPTNE PVIIKRLLDI GFYNFLIPFV ETKEEAEQAV ASTRYPPEGI 130 140 150 160 170 180 RGVSVSHRAN MFGTVADYFA QSNKNITILV QIESQQGVDN VDAIAATEGV DGIFVGPSDL 190 200 210 220 230 240 AAALGHLGNA SHPDVQKAIQ HIFNRASAHG KPSGILAPVE ADARRYLEWG ATFVAVGSDL 250 GVFRSATQKL ADTFKK

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG71198.1.
RefSeq	YP_671099.1.
3D structure databases
SMR	Q0TCY1. Positions 4-256.
ModBase	Search...
Genome annotation databases
GeneID	4188537.
GenomeReviews	Gene locus ECP_3216 in contig CP000247_GR.
KEGG	ecp:ECP_3216.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TCY1.
OMA	Q0TCY1. QRSNRYG.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_3216-MON.
Family and domain databases
HAMAP	MF_01291. [Tree]
InterPro	IPR017648. Dehyd-dGlucarate-aldolase_GarL. IPR005000. HpcH_HpaI. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF03328. HpcH_HpaI. 1 hit. [Graphical view]
ProDom	PD001009. Pyruvate_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03239. GarL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GARL_ECOL5

Accession

Primary (citable) accession number: Q0TCY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)