ID   KBAY_ECOL5              Reviewed;         286 AA.
AC   Q0TCW8;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=D-tagatose-1,6-bisphosphate aldolase subunit kbaY;
DE            Short=TagBP aldolase;
DE            Short=TBPA;
DE            EC=4.1.2.40;
DE   AltName: Full=Tagatose-bisphosphate aldolase;
DE   AltName: Full=D-tagatose-bisphosphate aldolase class II;
DE   AltName: Full=Ketose 1,6-bisphosphate aldolase class II;
GN   Name=kbaY; OrderedLocusNames=ECP_3229;
OS   Escherichia coli O6:K15:H31 (strain 536 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=362663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16879640; DOI=10.1111/j.1365-2958.2006.05255.x;
RA   Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U.,
RA   Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.;
RT   "Role of pathogenicity island-associated integrases in the genome
RT   plasticity of uropathogenic Escherichia coli strain 536.";
RL   Mol. Microbiol. 61:584-595(2006).
CC   -!- FUNCTION: Catalytic subunit of the tagatose-1,6-bisphosphate
CC       aldolase kbaYZ, which catalyzes the reversible aldol condensation
CC       of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with
CC       glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-
CC       bisphosphate (TBP). Requires kbaZ subunit for full activity and
CC       stability (By similarity).
CC   -!- CATALYTIC ACTIVITY: D-tagatose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate
CC       degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate
CC       from D-tagatose 6-phosphate: step 2/2.
CC   -!- SUBUNIT: Homotetramer. Forms a complex with kbaZ (By similarity).
CC   -!- SIMILARITY: Belongs to the class II fructose-bisphosphate aldolase
CC       family. TagBP aldolase kbaY subfamily.
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DR   EMBL; CP000247; ABG71211.1; -; Genomic_DNA.
DR   RefSeq; YP_671112.1; -.
DR   SMR; Q0TCW8; 2-284.
DR   GeneID; 4188585; -.
DR   GenomeReviews; CP000247_GR; ECP_3229.
DR   KEGG; ecp:ECP_3229; -.
DR   HOGENOM; Q0TCW8; -.
DR   OMA; Q0TCW8; TIELGVC.
DR   BioCyc; ECOL362663:ECP_3229-MON; -.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:InterPro.
DR   GO; GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-ph...; IEA:InterPro.
DR   HAMAP; MF_01293; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000771; Ketose_bisP_aldolase_II.
DR   InterPro; IPR011288; Tag_bisphos_ald.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01116; F_bP_aldolase; 1.
DR   PIRSF; PIRSF001359; F_bP_aldolase_II; 1.
DR   ProDom; PD002376; K_bP_aldolase; 1.
DR   TIGRFAMs; TIGR00167; cbbA; 1.
DR   TIGRFAMs; TIGR01858; tag_bisphos_ald; 1.
DR   PROSITE; PS00602; ALDOLASE_CLASS_II_1; 1.
DR   PROSITE; PS00806; ALDOLASE_CLASS_II_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    286       D-tagatose-1,6-bisphosphate aldolase
FT                                subunit kbaY.
FT                                /FTId=PRO_0000355327.
FT   REGION      209    211       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   REGION      230    233       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   ACT_SITE     82     82       Proton donor (By similarity).
FT   METAL        83     83       Zinc; catalytic (By similarity).
FT   METAL       180    180       Zinc; catalytic (By similarity).
FT   METAL       208    208       Zinc; catalytic (By similarity).
FT   BINDING     181    181       Dihydroxyacetone phosphate; via amide
FT                                nitrogen (By similarity).
SQ   SEQUENCE   286 AA;  31294 MW;  CAA42DF05C2B918B CRC64;
     MSIISTKYLL QDAQANGYAV PAFNIHNAET IQAILEVCSE MRSPVILAGT PGTFKHIALE
     EIYALCSAYS TTYNMPLALH LDHHESLDDI RRKVHAGVRS AMIDGSHFPF AENVKLVKSV
     VDFCHSQDCS VEAELGRLGG VEDDMSVDAE SAFLTDPQEA KRFVELTGVD SLAVAIGTAH
     GLYSKTPKID FQRLAEIREV VDVPLVLHGA SDVPDEFVRR TIELGVTKVN VATELKIAFA
     GAVKAWFAEN PQGNDPRYYM RVGMDAMKEV VRNKINVCGS ANRISA
//