Q0TCP4 (NANK_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmannosamine kinase
EC=2.7.1.60

Alternative name(s):
ManNAc kinase
N-acetyl-D-mannosamine kinase

Gene names

Name:	nanK
Ordered Locus Names:	ECP_3305

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P By similarity. HAMAP MF_01234
Catalytic activity	ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate. HAMAP MF_01234
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 2/5. HAMAP MF_01234
Subunit structure	Homodimer By similarity. HAMAP MF_01234
Sequence similarities	Belongs to the ROK (nagC/xylR) family. NanK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acylmannosamine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	N-acetylmannosamine kinase HAMAP MF_01234		PRO_0000301458
Regions
	Nucleotide binding	5 – 12	8	ATP Potential
	Nucleotide binding	132 – 139	8	ATP Potential
Sites
	Metal binding	156	1	Zinc By similarity
	Metal binding	166	1	Zinc By similarity
	Metal binding	168	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TCP4 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 2EA97FAB54B1D7B8	FASTA	291	29,685
	10 20 30 40 50 60 MTTLAIDIGG TKLAAALIGA DGQIRDRREL PTPASQTPQA LRDALSALVS PLQAHAQRVA 70 80 90 100 110 120 IASTGIIRDG SLLALNPHNL GGLLHFPLVK TLEQLTNLPT IAINDAQAAA WAEYQALDGD 130 140 150 160 170 180 ITDMVFITVS TGVGGGVVSG GKLRTGPGGL AGHIGHTLAD PHGPVCGCGR TGCVEAIASG 190 200 210 220 230 240 RGIATAAQGE LAGANAKTIF TRAGQGDEQA QQLIHRSART LARLIADIKA TTDCQCVVVG 250 260 270 280 290 GSVGLAEGYL ALVETYLAQE PAAFHVDLLA AHYRHDAGLL GAALLAQGEK L

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 536 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000247 Genomic DNA. Translation: ABG71285.1.
RefSeq	YP_671186.1. NC_008253.1.
3D structure databases
ProteinModelPortal	Q0TCP4.
SMR	Q0TCP4. Positions 1-289.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0TCP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000050335; EBESCP00000048524; EBESCG00000049383.
GeneID	4188825.
GenomeReviews	Gene locus ECP_3305 in contig CP000247_GR.
KEGG	ecp:ECP_3305.
NMPDR	fig|340197.3.peg.4535.
PATRIC	18197462. VBIEscCol77757_3348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1940.
GeneTree	EBGT00050000009665.
HOGENOM	HBG730260.
OMA	LRISQCR.
PhylomeDB	Q0TCP4.
ProtClustDB	PRK05082.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_3305-MONOMER.
Family and domain databases
HAMAP	MF_01234. ManNAc_kinase. [Tree]
InterPro	IPR023945. ManNAc_kinase_bac. IPR000600. ROK. [Graphical view]
KO	K00885.
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NANK_ECOL5

Accession

Primary (citable) accession number: Q0TCP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program