Q0TCG5 (RS4_ECOL5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S4

Gene names

Name:	rpsD
Ordered Locus Names:	ECP_3384

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit By similarity. HAMAP MF_01306_B With S5 and S12 plays an important role in translational accuracy By similarity. HAMAP MF_01306_B
Subunit structure	Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity By similarity.
Sequence similarities	Belongs to the ribosomal protein S4P family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 206

206

30S ribosomal protein S4 HAMAP MF_01306_B

PRO_0000293279

Regions

Domain

96 – 156

61

S4 RNA-binding

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

206

Helix Strand Turn

Details...

Helix

9 – 14

6

Beta strand

28 – 32

5

Beta strand

34 – 36

3

Helix

56 – 58

3

Helix

59 – 62

4

Helix

69 – 81

13

Helix

89 – 94

6

Helix

100 – 104

5

Helix

113 – 118

6

Turn

119 – 121

3

Beta strand

149 – 151

3

Helix

154 – 162

9

Helix

198 – 201

4

Sequences

	Sequence		Length	Mass (Da)
	Q0TCG5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 4015969DF8E582BB	FASTA	206	23,469
	10 20 30 40 50 60 MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK 70 80 90 100 110 120 VRRIYGVLER QFRNYYKEAA RLKGNTGENL LALLEGRLDN VVYRMGFGAT RAEARQLVSH 130 140 150 160 170 180 KAIMVNGRVV NIASYQVSPN DVVSIREKAK KQSRVKAALE LAEQREKPTW LEVDAGKMEG 190 200 TFKRKPERSD LSADINEHLI VELYSK

References

[1]	"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 536 / UPEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000247 Genomic DNA. Translation: ABG71364.1.

RefSeq

YP_671265.1. NC_008253.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VHO	X-ray	3.74	D	2-206	[»]

ProteinModelPortal

Q0TCG5.

SMR

Q0TCG5. Positions 2-206.

ModBase

Search...

Protein-protein interaction databases

STRING

Q0TCG5.

Proteomic databases

PRIDE

Q0TCG5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000047824; EBESCP00000046013; EBESCG00000046874.

GeneID

4191210.

GenomeReviews

Gene locus ECP_3384 in contig CP000247_GR.

KEGG

ecp:ECP_3384.

PATRIC

18197624. VBIEscCol77757_3423.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0522.

GeneTree

EBGT00050000010511.

HOGENOM

HBG285740.

OMA

QLVVELY.

PhylomeDB

Q0TCG5.

ProtClustDB

PRK05327.

Enzyme and pathway databases

BioCyc

ECOL362663:ECP_3384-MONOMER.

Family and domain databases

HAMAP

MF_01306_B. Ribosomal_S4_B.
[Tree]

InterPro

IPR022801. Ribosomal_S4/S9.
IPR001912. Ribosomal_S4/S9_N.
IPR005709. Ribosomal_S4_bac-type.
IPR018079. Ribosomal_S4_CS.
IPR002942. S4_RNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.1050.10. G3DSA:1.10.1050.10. 1 hit.
G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit.

KO

K02986.

PANTHER

PTHR11831. Ribosomal_S4. 1 hit.

Pfam

PF00163. Ribosomal_S4. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]

SMART

SM00363. S4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01017. RpsD_bact. 1 hit.

PROSITE

PS00632. RIBOSOMAL_S4. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS4_ECOL5

Accession

Primary (citable) accession number: Q0TCG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program