Reviewed, UniProtKB/Swiss-Prot Q0TC64 (PCKA_ECOL5)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxykinase [ATP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.49
Alternative name(s):
    Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	ECP_3489

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453		PRO_1000026324
Regions
	Nucleotide binding	248 – 255	8	ATP By similarity
Amino acid modifications
	Modified residue	87	1	N6-acetyllysine By similarity
	Modified residue	523	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TC64-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C71ABF697812FA2C	FASTA	540	59,615
	10 20 30 40 50 60 MRVNNGLTPQ ELEAYGISDV HDIVYNPSYD LLYQEELDPS LTGYERGVLT NLGAVAVDTG 70 80 90 100 110 120 IFTGRSPKDK YIVRDDTTRD TFWWADKGKG KNDNKPLSPE TWQHLKGLVT KQLSGKRLFV 130 140 150 160 170 180 VDAFCGANPD TRLSVRFITE VAWQAHFVKN MFIRPSDEEL AGFKPDFIVM NGAKCTNPQW 190 200 210 220 230 240 KEQGLNSENF VAFNLTERMQ LIGGTWYGGE MKKGMFSMMN YLLPLKGIAS MHCSANVGEK 250 260 270 280 290 300 GDVAVFFGLS GTGKTTLSTD PKRRLIGDDE HGWDDDGVFN FEGGCYAKTI KLSKEAEPEI 310 320 330 340 350 360 YNAIRRDALL ENVTVREDGT IDFDDGSKTE NTRVSYPIYH IDNIVKPVSK AGHATKVIFL 370 380 390 400 410 420 TADAFGVLPP VSRLTADQTQ YHFLSGFTAK LAGTERGITE PTPTFSACFG AAFLSLHPTQ 430 440 450 460 470 480 YAEVLVKRMQ AAGAQAYLVN TGWNGTGKRI SIKDTRAIID AILNGSLDNA ETFTLPMFNL 490 500 510 520 530 540 AIPTELPGVD TKILDPRNTY ASPEQWQEKA ETLAKLFIDN FDKYTDTPAG AALVAAGPKL

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG71465.1.
RefSeq	YP_671366.1.
3D structure databases
SMR	Q0TC64. Positions 10-539.
ModBase	Search...
Genome annotation databases
GeneID	4191340.
GenomeReviews	Gene locus ECP_3489 in contig CP000247_GR.
KEGG	ecp:ECP_3489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TC64.
OMA	Q0TC64. MFIRPTE.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_3489-MON.
Family and domain databases
HAMAP	MF_00453. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
ProDom	PD004738. PEPCK_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_ECOL5

Accession

Primary (citable) accession number: Q0TC64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)