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Q0TC55

- BIOH_ECOL5

UniProt

Q0TC55 - BIOH_ECOL5

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Protein
Pimeloyl-[acyl-carrier protein] methyl ester esterase
Gene
bioH, ECP_3498
Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei82 – 821Nucleophile By similarity
Active sitei207 – 2071 By similarity
Active sitei235 – 2351 By similarity
Binding sitei235 – 2351Substrate By similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciECOL362663:GIY5-3528-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esterase (EC:3.1.1.85)
Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene namesi
Name:bioH
Ordered Locus Names:ECP_3498
OrganismiEscherichia coli O6:K15:H31 (strain 536 / UPEC)
Taxonomic identifieri362663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000009182: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation
PRO_1000067267Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi362663.ECP_3498.

Structurei

3D structure databases

ProteinModelPortaliQ0TC55.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate binding By similarity
Regioni143 – 1475Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiANICHHI.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TC55-1 [UniParc]FASTAAdd to Basket

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MNNIWWQTKG QGNVHLVLLH GWGLNAEVWR CIDEELSSHF TLHLVDLPGF    50
GRSRGFGALS LADMAEVVLR QAPDKAIWLG WSLGGLVASQ IALTHPERVL 100
ALVTVASSPC FSARDEWPGI KPDVLAGFQQ QLSDDFQRTV ERFLALQTMG 150
TETARQDARA LKKTVLALPM PEVDVLNGGL EILKTVDLRL PLQNVSMPFL 200
RLYGYLDGLV PRKVVPMLDK LWPHSESYIF AKAAHAPFIS HPVEFHHLLV 250
ALKQRV 256
Length:256
Mass (Da):28,593
Last modified:September 5, 2006 - v1
Checksum:i24667BD9ADBDA8F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG71474.1.
RefSeqiYP_671375.1. NC_008253.1.

Genome annotation databases

EnsemblBacteriaiABG71474; ABG71474; ECP_3498.
GeneIDi4191349.
KEGGiecp:ECP_3498.
PATRICi18197856. VBIEscCol77757_3539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG71474.1 .
RefSeqi YP_671375.1. NC_008253.1.

3D structure databases

ProteinModelPortali Q0TC55.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362663.ECP_3498.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG71474 ; ABG71474 ; ECP_3498 .
GeneIDi 4191349.
KEGGi ecp:ECP_3498.
PATRICi 18197856. VBIEscCol77757_3539.

Phylogenomic databases

eggNOGi COG0596.
HOGENOMi HOG000028062.
KOi K02170.
OMAi ANICHHI.
OrthoDBi EOG6FJNH9.

Enzyme and pathway databases

UniPathwayi UPA00078 .
BioCyci ECOL362663:GIY5-3528-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_01260. Carboxylester.
InterProi IPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
TIGRFAMsi TIGR01738. bioH. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
    Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
    Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 536 / UPEC.

Entry informationi

Entry nameiBIOH_ECOL5
AccessioniPrimary (citable) accession number: Q0TC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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