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Q0TC55

- BIOH_ECOL5

UniProt

Q0TC55 - BIOH_ECOL5

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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei82 – 821NucleophileUniRule annotation
Active sitei207 – 2071UniRule annotation
Active sitei235 – 2351UniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciECOL362663:GIY5-3528-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:ECP_3498
OrganismiEscherichia coli O6:K15:H31 (strain 536 / UPEC)
Taxonomic identifieri362663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000009182: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000067267Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi362663.ECP_3498.

Structurei

3D structure databases

ProteinModelPortaliQ0TC55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingUniRule annotation
Regioni143 – 1475Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiANICHHI.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0TC55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNIWWQTKG QGNVHLVLLH GWGLNAEVWR CIDEELSSHF TLHLVDLPGF
60 70 80 90 100
GRSRGFGALS LADMAEVVLR QAPDKAIWLG WSLGGLVASQ IALTHPERVL
110 120 130 140 150
ALVTVASSPC FSARDEWPGI KPDVLAGFQQ QLSDDFQRTV ERFLALQTMG
160 170 180 190 200
TETARQDARA LKKTVLALPM PEVDVLNGGL EILKTVDLRL PLQNVSMPFL
210 220 230 240 250
RLYGYLDGLV PRKVVPMLDK LWPHSESYIF AKAAHAPFIS HPVEFHHLLV

ALKQRV
Length:256
Mass (Da):28,593
Last modified:September 5, 2006 - v1
Checksum:i24667BD9ADBDA8F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG71474.1.
RefSeqiYP_671375.1. NC_008253.1.

Genome annotation databases

EnsemblBacteriaiABG71474; ABG71474; ECP_3498.
GeneIDi4191349.
KEGGiecp:ECP_3498.
PATRICi18197856. VBIEscCol77757_3539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000247 Genomic DNA. Translation: ABG71474.1 .
RefSeqi YP_671375.1. NC_008253.1.

3D structure databases

ProteinModelPortali Q0TC55.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362663.ECP_3498.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG71474 ; ABG71474 ; ECP_3498 .
GeneIDi 4191349.
KEGGi ecp:ECP_3498.
PATRICi 18197856. VBIEscCol77757_3539.

Phylogenomic databases

eggNOGi COG0596.
HOGENOMi HOG000028062.
KOi K02170.
OMAi ANICHHI.
OrthoDBi EOG6FJNH9.

Enzyme and pathway databases

UniPathwayi UPA00078 .
BioCyci ECOL362663:GIY5-3528-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_01260. Carboxylester.
InterProi IPR029058. AB_hydrolase.
IPR010076. BioH.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
TIGRFAMsi TIGR01738. bioH. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
    Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
    Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 536 / UPEC.

Entry informationi

Entry nameiBIOH_ECOL5
AccessioniPrimary (citable) accession number: Q0TC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3