Reviewed, UniProtKB/Swiss-Prot Q0TBG6 (RNPH_ECOL5)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	ECP_3741

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024803

Sequences

	Sequence		Length	Mass (Da)
	Q0TBG6-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C73A74F3ECC8560E	FASTA	238	25,340
	10 20 30 40 50 60 MRPAGRSNNQ VRPVTLTRNY TKHAEGSVLV EFGDTKVLCT ASIEEGVPRF LKGQGQGWIT 70 80 90 100 110 120 AEYGMLPRST HTRNAREAAK GKQGGRTMEI QRLIARALRA AVDLKALGEF TITLDCDVLQ 130 140 150 160 170 180 ADGGTRTASI TGACVALADA LQKLVENGKL KTNPMKGMVA AVSVGIVNGE AVCDLEYVED 190 200 210 220 230 SAAETDMNVV MTEDGRIIEV QGTAEGEPFT HEELLTLLAL ARGGIESIVV TQKAALAN

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG71713.1.
RefSeq	YP_671614.1.
3D structure databases
SMR	Q0TBG6. Positions 2-237.
ModBase	Search...
Genome annotation databases
GeneID	4189314.
GenomeReviews	Gene locus ECP_3741 in contig CP000247_GR.
KEGG	ecp:ECP_3741.
NMPDR	fig|340197.3.peg.526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TBG6.
OMA	Q0TBG6. YAMLPRA.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_3741-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_ECOL5

Accession

Primary (citable) accession number: Q0TBG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)