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Protein

Acetolactate synthase

Gene

ECP_3878

Organism
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. acetolactate synthase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  2. valine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Thiamine pyrophosphateUniRule annotation

Enzyme and pathway databases

BioCyciECOL362663:GIY5-3907-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthaseUniRule annotation (EC:2.2.1.6UniRule annotation)
Gene namesi
Ordered Locus Names:ECP_3878Imported
OrganismiEscherichia coli O6:K15:H31 (strain 536 / UPEC)Imported
Taxonomic identifieri362663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000009182: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi362663.ECP_3878.

Structurei

3D structure databases

ProteinModelPortaliQ0TB30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0028.
HOGENOMiHOG000258448.
KOiK01652.
OMAiCMASSGP.
OrthoDBiEOG6KT2NW.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0TB30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSGTTSTR KRFTGAEFIV HFLEQQGIKI VTGIPGGSIL PVYDALSQST
60 70 80 90 100
QIRHILARHE QGAGFIAQGM ARTDGKPAVC MACSGPGATN LVTAIADARL
110 120 130 140 150
DSIPLICITG QVSASMIGTD AFQEVDTYGI SIPITKHNYL VRHIEELPQV
160 170 180 190 200
MSDAFRIAQS GRPGPVWIDI PKDVQTAVFE IETQPAMAEK AAAPAFSEES
210 220 230 240 250
IRDAAAMINA AKRPVLYLGG GVINAPARVR ELAEKAQLPT TMTLMALGML
260 270 280 290 300
PKAHPLSLGM LGMHGVRSTN YILQEADLLI VLGARFDDRA IGKTEQFCPN
310 320 330 340 350
AKIIHVDIDR AELGKIKQPH VAIQADVDDV LAQLIPLVEA QPRAEWHQLV
360 370 380 390 400
ADLQREFPCP IPKACDPLSH YGLINAVAAC VDDNAIITTD VGQHQMWTAQ
410 420 430 440 450
AYPLNRPRQW LTSGGLGTMG FGLPAAIGAA LANPDRKVLC FSGDGSLMMN
460 470 480 490 500
IQEMATASEN QLDVKIILMN NEALGLVHQQ QSLFYKQGVF AATYPGKINF
510 520 530 540 550
MQIAAGFGLE TCDLNNEADP QAALQEIINR PGPALIHVRI DAEEKVYPMV
560
PPGAANTEMV GE
Length:562
Mass (Da):60,414
Last modified:September 5, 2006 - v1
Checksum:i3112559DCE74DEB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000247 Genomic DNA. Translation: ABG71849.1.
RefSeqiYP_671750.1. NC_008253.1.

Genome annotation databases

EnsemblBacteriaiABG71849; ABG71849; ECP_3878.
GeneIDi4189067.
KEGGiecp:ECP_3878.
PATRICi18198620. VBIEscCol77757_3918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000247 Genomic DNA. Translation: ABG71849.1.
RefSeqiYP_671750.1. NC_008253.1.

3D structure databases

ProteinModelPortaliQ0TB30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi362663.ECP_3878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG71849; ABG71849; ECP_3878.
GeneIDi4189067.
KEGGiecp:ECP_3878.
PATRICi18198620. VBIEscCol77757_3918.

Phylogenomic databases

eggNOGiCOG0028.
HOGENOMiHOG000258448.
KOiK01652.
OMAiCMASSGP.
OrthoDBiEOG6KT2NW.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.
BioCyciECOL362663:GIY5-3907-MONOMER.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
    Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
    Mol. Microbiol. 61:584-595(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 536 / UPECImported.

Entry informationi

Entry nameiQ0TB30_ECOL5
AccessioniPrimary (citable) accession number: Q0TB30
Entry historyi
Integrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: February 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.