Reviewed, UniProtKB/Swiss-Prot Q0TAA4 (CAPP_ECOL5)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxylase
      Short name=PEPCase
      Short name=PEPC
    EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	ECP_4169

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	883 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. HAMAP MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + CO2. HAMAP MF_00595
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00595
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Tricarboxylic acid cycle
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization by fixation of carbon dioxide Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 883	883	Phosphoenolpyruvate carboxylase HAMAP MF_00595		PRO_1000025557
Sites
	Active site	138	1	By similarity
	Active site	546	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0TAA4-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C66E18268EB19EFD	FASTA	883	99,077
	10 20 30 40 50 60 MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILERVETIRK LSKSSRAGND ANRQELLTTL 70 80 90 100 110 120 QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN PEVIARTLRK LKNQPELSED 130 140 150 160 170 180 TIKKAVESLS LELVLTAHPT EITRRTLIHK MVEVNACLKQ LDNKDIADYE HNQLMRRLRQ 190 200 210 220 230 240 LIAQSWHTDE IRKLRPSPVD EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVEF 250 260 270 280 290 300 VPVRFTSWMG GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIQVLVSEL SMVEATPELL 310 320 330 340 350 360 ALVGEEGAAE PYRYLMKNLR SRLMATQAWL EARLKGEELP KPEGLLTQNE ELWEPLYACY 370 380 390 400 410 420 QSLQACGMGI IANGDLLDTL RRVKCFGVPL VRIDIRQEST RHTEALGELT RYLGIGDYES 430 440 450 460 470 480 WSEADKQAFL IRELNSKRPL LPRNWQPSAE TREVLDTCQV IAEAPQGSIA AYVISMAKTP 490 500 510 520 530 540 SDVLAVHLLL KEAGIGFAMP VAPLFETLDD LNNANDVMTQ LLNIDWYRGL IQGKQMVMIG 550 560 570 580 590 600 YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP 610 620 630 640 650 660 PGSLKGGLRV TEQGEMIRFK YGLPEITVSS LSLYTGAILE ANLLPPPEPK ESWRRIMDEL 670 680 690 700 710 720 SVISCDLYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP AKRRPTGGVE SLRAIPWIFA 730 740 750 760 770 780 WTQNRLMLPA WLGAGTALQK VVEDGKQSEL EAMCRDWPFF STRLGMLEMV FAKADLWLAE 790 800 810 820 830 840 YYDQRLVDKA LWPLGKELRN LQEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN 850 860 870 880 VLQAELLHRS RQAEKEGQEP DPRVEQALMV TIAGIAAGMR NTG

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG72125.1.
RefSeq	YP_672026.1.
3D structure databases
SMR	Q0TAA4. Positions 4-883.
ModBase	Search...
Genome annotation databases
GeneID	4189197.
GenomeReviews	Gene locus ECP_4169 in contig CP000247_GR.
KEGG	ecp:ECP_4169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0TAA4.
OMA	Q0TAA4. KITEQGE.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_4169-MON.
Family and domain databases
HAMAP	MF_00595. [Tree]
InterPro	IPR001449. PEP_COase. IPR018129. PEP_COase_AS. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_ECOL5

Accession

Primary (citable) accession number: Q0TAA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)